The interactome of Musclin on the membrane of adipocytes
| Title | The interactome of Musclin on the membrane of adipocytes |
|---|---|
| Description | A proximity-dependent biotin identification (BioID) assay was used to identify the interactome for Musclin on the plasma membrane of adipocytes. pCMV-BirA* (Control), pCMV-Musclin-BirA* plasmids were transfected into the HEK293T cells. After transfection for 12 h, cell medium-enriched in BirA* or Musclin-BirA* fusion protein were collected for incubation of C3H10T1/2-derived adipocytes, supplemented with 80 μM biotin for 24 h. The membrane-associated proteins from adipocytes were separated and were then pulled down using streptavidin agarose. The agarose beads were pelleted for obtaining Musclin-interacted proteins, followed by denaturation, silver staining and M.S. analysis. |
| Organism | Mus musculus |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA028709 |
| Release Date | 2024-09-01 |
| Submitter | Zhuoxian Meng (zxmeng@zju.edu.cn) |
| Organization | Zhejiang Univeristy |
| Submission Date | 2024-07-31 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX007040-01 | The interactome of Musclin on the membrane of adipocytes | 1 | Proteomic Data by Mass Spectrometry (MS) | 380.8 KB | xlsx | 0 |