| Title | High fructose consumption aggravates inflammation by promoting Th1 and Th17 cell generations through the glutamine metabolism-dependent mTORC1 activation |
|---|---|
| Description | To investigate how fructose regulates Th1 and Th17 cell differentiation, we used bulk RNA sequencing (RNA-seq). Complete DMEM (cDMEM) was prepared for all cell cultures by adding 25 mM glucose or fructose to the glucose-free DMEM. CD4+CD25-CD62L+ naive T cells isolated from C57BL/6 mice were cultured in 48-well plates (2 x 105 per well) in the presence of plate-bound anti-mouse CD3 (1.5 ug/mL) and soluble anti-mouse CD28 (1.5 ug/mL), mouse IL-6 (50 ng/mL), TGF-β1 (2 ng/mL), and mouse IL-12 (10 ng/mL). Cells were cultured at 37°C, 5% CO2, and collected for sequencing analysis three days later. |
| Organism | Mus musculus |
| Data Type | Metabolome Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA029873 |
| Release Date | 2025-06-07 |
| Submitter | Fang Nan (IRITS_Lab@outlook.com) |
| Organization | Sichuan University |
| Submission Date | 2024-09-09 |
HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client).
| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download |
|---|---|---|---|---|---|---|
| OMIX007324-01 | T cells, 25 mM glucose or fructose cDMEM treated, 3Day | 44 | Metabolome Data by Mass Spectrometry (MS) | 666.57 MB | zip |
| Paper Title | Journal Name | Publish Time | Accession | Citing Type |
|---|---|---|---|---|
| High fructose consumption aggravates inflammation by promoting effector T cell generation via inducing metabolic reprogramming | Signal Transduction and Targeted Therapy | 2025-08 | OMIX007324 | Deposit |