| Title |
Microbial tyrosine metabolites play a central role in mediating the anti-colitis effect of palmatine |
| Description |
Colonic tissue (30 mg) was transferred to homogenizer (pre-cooled at -20 °C) and 100 µl of H2O (pre-cooled at 4 °C) was added for homogenate for 1 min. 800 µL of ice-cold methanol solution containing 1 μg/ml 4-chloro-phenylalanine was added for protein precipitation and vortexed for 10 min. The sample lysates were centrifuged at 18000 rpm for 10 min at 4 °C, and dried under vacuum. Metabolomic study was performed on the LC/Q-TOF-MS (Agilent 6546). A Waters Atlantis T3 and a XBridge BEH Amide column were used for negative analyses. |
| Organism |
Mus |
| Data Type |
Metabolome Data by Mass Spectrometry (MS) |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA032940 |
| Release Date |
2025-05-01 |
| Submitter |
Daming Sun (sundm128@163.com) |
| Organization |
China Pharmaceutical University |
| Submission Date |
2024-11-29 |