| Title |
Processed data of transcriptomic analysis for E15.5 female germ cells |
| Description |
Raw RNA-seq reads were processed for quality assessment using FastQC (v0.12.1) and trimmed to remove adapters and low-quality bases with Trim Galore (v0.6.10) under default parameters. HISAT2 (v2.21) was used to map the trimmed reads to the mouse reference genome (GRCm39), which was downloaded from the ENCODE database, using default parameters. Stringtie (v2.2.1) was used for gene expression quantification, and the resulting transcript quantification files were merged into a unified gene expression matrix using the prepDE.py script. Differential expression analysis was conducted using DESeq2 (v1.38.3). |
| Organism |
Mus musculus |
| Data Type |
Expression Profiling by NGS |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA033553 |
| Release Date |
2025-06-19 |
| Submitter |
Yi Na Zhang (zhangyina22@mails.ucas.ac.cn) |
| Organization |
Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology |
| Submission Date |
2024-12-30 |