| Description |
Collect fecal samples from five healthy adult volunteers, homogenize them, and inoculate them into five types of solid media and ten types of liquid media. These samples were cultured in anaerobic chambers with and without CO2. DNA was extracted from the initial fecal mixtures and the cultures, followed by metagenomic sequencing. For all metagenomic sequencing data, fastp was used to filter out low-quality sequences and adapter sequences. To remove host sequences from the fecal samples, the human reference genome (GRCh38.p14) was downloaded. The KneadData tool was then used to remove host sequences. After quality control, clean reads were used to calculate the relative abundance of microbial species using MetaPhlAn3. To characterize the general metabolic and functional composition of our metagenomic sequencing data, the clean reads were analyzed by using HUMAnN3. |