| Title |
Gut microbiota alleviates immune response during E. coli-induced bladder cystitis via AhR/Prg4 activation |
| Description |
Metabolomic analysis was performed using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry following established protocols. Quality control samples were prepared by pooling equal volumes of each AH sample to assess the stability and reproducibility of the untargeted metabolic analysis. The current untargeted metabolic analysis was performed via GC-MS and LC-MS. For GC analysis, AH samples were analyzed on an Agilent 7890B gas chromatograph coupled to an Agilent 5977B MSD system, and an HP-5MS fused-silica capillary column was utilized to separate the derivatives. For LC-MS, an ACQUITY UPLC I-Class system coupled with a VION IMS QTOF Mass spectrometer was used to analyze the metabolic profiling in both ESI positive and ESI negative ion modes. |
| Organism |
Mus |
| Data Type |
Metabolome Data by Mass Spectrometry (MS) |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA034856 |
| Release Date |
2025-01-13 |
| Submitter |
Yang Yang (yangyilcyl@163.com) |
| Organization |
The First Affiliated Hospital of Anhui Medical University |
| Submission Date |
2025-01-10 |