| Title | RBM25 is required to restrain inflammation via ACLY RNA splicing-dependent metabolism rewiring |
|---|---|
| Description | BMDMs (1 x 107 cells) were used to perform a metabolomics assay at Shanghai Biotree Biotech Co. (China). Briefly, for each sample, 500 μl of precooled MeOH/H2O (3/1, v/v) was added. The samples were then vortexed for 30 sec. After being precooled on dry ice, the samples were frozen and thawed three times in liquid nitrogen. The samples were then vortexed for 30 sec and sonicated for 15 min in an ice water bath. Next, as a subsequent step, the samples were incubated at -40 °C for 1 h and centrifuged at 12000 rpm for 15 min at 4 °C. Afterwards, an aliquot of 400 μl of clear supernatant was collected and dried by spinning. As a reconstitution solution, 200 ml of ultrapure water was mixed with the residue. The reconstituted samples were vortexed before passing through the filter of the centrifuge tube, after which they were transferred to inserts in injection vials for HPLC-MS/MS analysis. |
| Organism | Mus musculus |
| Data Type | Metabolome Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA034691 |
| Release Date | 2025-12-31 |
| Submitter | Bo Wang (wangb1003@126.com) |
| Organization | Tongji University |
| Submission Date | 2025-01-15 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download |
|---|---|---|---|---|---|---|
| OMIX008695-01 | WT1 | 1 | Metabolome Data by Mass Spectrometry (MS) | 214.7 KB | zip | |
| OMIX008695-02 | WT2 | 1 | Metabolome Data by Mass Spectrometry (MS) | 217.7 KB | zip | |
| OMIX008695-03 | WT3 | 1 | Metabolome Data by Mass Spectrometry (MS) | 217.3 KB | zip | |
| OMIX008695-04 | WT4 | 1 | Metabolome Data by Mass Spectrometry (MS) | 217.4 KB | zip | |
| OMIX008695-05 | WT5 | 1 | Metabolome Data by Mass Spectrometry (MS) | 215.5 KB | zip | |
| OMIX008695-06 | KO1 | 1 | Metabolome Data by Mass Spectrometry (MS) | 214.8 KB | zip | |
| OMIX008695-07 | KO2 | 1 | Metabolome Data by Mass Spectrometry (MS) | 217.2 KB | zip | |
| OMIX008695-08 | KO3 | 1 | Metabolome Data by Mass Spectrometry (MS) | 217.7 KB | zip | |
| OMIX008695-09 | KO4 | 1 | Metabolome Data by Mass Spectrometry (MS) | 214.6 KB | zip | |
| OMIX008695-10 | KO5 | 1 | Metabolome Data by Mass Spectrometry (MS) | 217.4 KB | zip |
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