| Title | Proteomic and functional analyses reveal the stress tolerance network in Saccharomyces boulardii during gastrointestinal challenge |
|---|---|
| Description | Cells were harvested and lysed in 300 µL ice-cold lysis buffer using sonication. The lysates were clarified by dual centrifugation to remove debris. Protein concentration was determined via BCA assay using a microplate reader, with standard curve interpolation for quantification. Aliquots were stored at -80°C until use. For SDS-PAGE analysis, 10 µg protein/sample was resolved on 12% gels, stained, and imaged. For proteomic processing, 100 µg protein was denatured in 120 µL reduction buffer, alkylated with 50 mM iodoacetamide, and purified via 10 kDa ultrafiltration. Filters were washed twice with 300 mM TEAB before tryptic digestion (1 µg trypsin/sample, 37°C, 12 h). Peptides were eluted with 200 mM TEAB, lyophilized, and stored at -80°C. |
| Organism | Saccharomyces |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA043139 |
| Release Date | 2025-07-16 |
| Submitter | Dingkang Wang (532971542@qq.com) |
| Organization | Xian Jiaotong University |
| Submission Date | 2025-07-14 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download |
|---|---|---|---|---|---|---|
| OMIX010982-01 | raw data | 12 | Proteomic Data by Mass Spectrometry (MS) | 42.91 GB | zip |
| Paper Title | Journal Name | Publish Time | Accession | Citing Type |
|---|---|---|---|---|
| Proteomic and Functional Analyses Reveal the Stress Tolerance Network in Saccharomyces boulardii during Gastrointestinal Challenge | Journal of Proteome Research | 2025-11 | OMIX010982 | Deposit |