| Description |
Panc-1 cells were treated with MOF-Fe and/or C20U4V for 48 h, homogenized in a mixture of chloroform/methanol/H2O (v/v/v = 3/6/1), and incubated at 1500 RPM for 1 h at 4 °C. Lipids were twice extracted from the mixture using chloroform, and the combined organic phase was pooled and dried in a SpeedVac under OH mode. The aqueous phase and cell pellet were also dried in a SpeedVac vacuum concentrator under H2O mode for protein quantification. Lipidomics analyses were conducted using an ExionLC-AD-Sciex QTRAP 6500 PLUS. Oxidized phospholipids were separated by normal phase-HPLC using a TUP-HB silica column (150 mm x 2.1 mm, 3 μm) with the following conditions: mobile phase A = chloroform/methanol/ammonium hydroxide = 89.5/10/0.5, mobile phase B = chloroform/methanol/ammonium hydroxide/water = 55/39/0.5/5.5. Individual lipid species were quantified by referencing to spiked internal standards. |