Evaluation of liver tissue extraction protocol for untargeted metabolic profiles by UPLC-Q-TOF-MS
| Title | Evaluation of liver tissue extraction protocol for untargeted metabolic profiles by UPLC-Q-TOF-MS |
|---|---|
| Description | The aim of the untargeted metabolic profiling study is to obtain a global metabolome coverage from biological samples. Therefore, a comprehensive and systematic protocol for tissue metabolite extraction is highly desirable. In this study, we evaluated a comprehensive liver pretreatment strategy based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) to expand metabolome coverage using four different protocols. These protocols included (A) methanol (MeOH) protein precipitation, (B) two-step extraction of dichloromethane (DCM)/MeOH followed by MeOH/H2O, (C) two-step extraction of methyl tert-butyl ether (MTBE)/MeOH followed by MeOH/H2O, and (D) two-step extraction of isopropanol (IPA)/MeOH followed by MeOH/H2O. |
| Organism | Rattus norvegicus |
| Data Type | - |
| Data Accessibility | Open-access |
| BioProject | PRJCA003762 |
| Release Date | 2020-10-28 |
| Submitter | Ying Li (liying_helen@163.com) |
| Organization | Harbin Medical University |
| Submission Date | 2020-10-28 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX210-30-01 | samples description | 1 | Metabonomics | 15.6 KB | xlsx | 42 | |
| OMIX210-30-02 | data in negative mode | 21 | Metabonomics | 17.8 GB | rar | 131 | |
| OMIX210-30-03 | data in positive mode | 21 | Metabonomics | 15.5 GB | rar | 130 |