| Description |
Using Tandem mass spectrometry (TMT)-labeled quantitative proteomics, we established a comprehensive proteome database of canines' brain by comparing protein expression levels in three age groups (newborns, 3 months and adults) in six brain regions (hippocampus, cerebellum, prefrontal cortex, olfactory bulb and striatum). We found a more substantial difference in proteome between newborn and 3-month puppy brain than that between 3-month and adult brain. We report that dogs isolated for 4 weeks 62 days after birth showed increased functional connectivity by functional MRI and decreased myelination in the prefrontal cortex by RNA seq and proteome analysis together with immunochemical analysis. These findings provide an omics and neural connectivity context to understand the consequences of SI and offer novel targets to develop therapies against SI-induced brain damage. |