| Accession |
PRJCA004289 |
| Title |
Social Isolation affects the proteome of brain |
| Relevance |
Model organism |
| Data types |
Transcriptome or Gene expression
Raw sequence reads
|
| Organisms |
Canis lupus familiaris
|
| Description |
Humans are social for well-being, prosperity and reproduction. Social isolation (SI) results in behavioral and cognitive dysfunction that correlates with white matter alteration in the brain. However, it remains unclear what aspects of oligodendrocyte involved myelination are regulated by SI and which molecular mechanisms underlie the events. Rodents have been widely used to study SI, but it is not known to what extent the knowledge we learned from rodents can be applicable in humans. Dogs have a higher genetic and brain anatomy similarity to humans than rodents and they show close cross-species social bond with humans. We sought to use dogs as a potential animal model for studying the effect of SI on brain development and function. Using Tandem mass spectrometry (TMT)-labeled quantitative proteomics, we established a comprehensive proteome database of canines' brain by comparing protein expression levels in three age groups (newborns, 3 months and adults) in six brain regions (hippocampus, cerebellum, prefrontal cortex, olfactory bulb and striatum). We found a more substantial difference in proteome between newborn and 3-month puppy brain than that between 3-month and adult brain. We report that dogs isolated for 4 weeks 62 days after birth showed increased functional connectivity by functional MRI and decreased myelination in the prefrontal cortex by RNA seq and proteome analysis together with immunochemical analysis. These findings provide an omics and neural connectivity context to understand the consequences of SI and offer novel targets to develop therapies against SI-induced brain damage. |
| Sample scope |
Monoisolate |
| Release date |
2021-05-31 |
| Data provider |
|
| Biomaterial provider |
Sinogene Biotechnology |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
Key Program
|
31830036
|
|
| National Natural Science Foundation of China (NSFC)
|
|
31921002
|
|
| National Key Research and Development Program
|
|
2019YFA0707100
|
|
| Chinese Academy of Sciences (CAS)
|
Strategic Priority Research Program of Chinese Academy of Sciences (CAS)
|
XDBS1020100
|
|
| Beijing Municipal Science & Technology Commission
|
|
Z181100001518001
|
|
|
|
| Submitter |
Qing
Yong
Zhang (yqzhang@genetics.ac.cn)
|
| Organization |
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
| Submission date |
2021-01-18 |
