OMIX

The chromatin accessibility landscape of human primordial germ cell-like cells

OMIX346

1Summary
Title The chromatin accessibility landscape of human primordial germ cell-like cells
Description In vitro induction of human primordial germ cell-like cells (hPGCLCs) provides an ideal platform to recapitulate hPGC development. However, the detailed molecular mechanisms regulating the induction of hPGCLCs remain largely uncharacterized. Here, we profiled the chromatin accessibility and transcriptome dynamics throughout the process of hPGCLC induction. Genetic ablation of SOX15 indicated the crucial roles of SOX15 in the maintenance of hPGCLCs. Mechanistically, SOX15 exerted its roles via suppressing somatic gene expression and sustaining latent pluripotency. Finally, a stepwise switch of OCT4/SOX2, OCT4/SOX17, and OCT4/SOX15 binding motifs were found to be enriched in closed-to-open regions of human embryonic stem cells, and early- and late-stage hPGCLCs, respectively. Collectively, our data characterized the chromatin accessibility and transcriptome landscapes throughout hPGCLC induction and defined the SOX15-mediated regulatory networks underlying this process.
Organism Homo sapiens
Data Type -
Data Accessibility Open-access
BioProject PRJCA005395
Release Date 2022-02-28
Submitter Xiaoyang Zhao (zhaoxiaoyang@smu.edu.cn)
Organization Southern Medical University
Submission Date 2021-06-09
2Files & Download

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OMIX346-99-01 subHRA001360.Suppl.tar 32 DNA Methylation 5.4 GB tar 16

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