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Emerging microbes & infections
Dec, 2020;9 (1): 761-770.

Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients.

Yong Xiong, Yuan Liu, Liu Cao, Dehe Wang, Ming Guo, Ao Jiang, Dong Guo, Wenjia Hu, Jiayi Yang, Zhidong Tang, Honglong Wu, Yongquan Lin, Meiyuan Zhang, Qi Zhang, Mang Shi, Yingle Liu, Yu Zhou, Ke Lan, Yu Chen
Author Information
  1. Yong Xiong: State Key Laboratory of Virology, Department of Infectious Disease, Zhongnan Hospital, Wuhan University, Wuhan, People's Republic of China.
  2. Yuan Liu: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
  3. Liu Cao: The Centre for Infection and Immunity Studies, School of Medicine, Sun Yat-sen University, Guangzhou, People's Republic of China.
  4. Dehe Wang: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
  5. Ming Guo: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
  6. Ao Jiang: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
  7. Dong Guo: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
  8. Wenjia Hu: State Key Laboratory of Virology, Department of Infectious Disease, Zhongnan Hospital, Wuhan University, Wuhan, People's Republic of China.
  9. Jiayi Yang: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
  10. Zhidong Tang: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
  11. Honglong Wu: BGI PathoGenesis Pharmaceutical Technology, Shenzhen, People's Republic of China.
  12. Yongquan Lin: BGI PathoGenesis Pharmaceutical Technology, Shenzhen, People's Republic of China. ORCID
  13. Meiyuan Zhang: BGI PathoGenesis Pharmaceutical Technology, Shenzhen, People's Republic of China.
  14. Qi Zhang: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China. ORCID
  15. Mang Shi: The Centre for Infection and Immunity Studies, School of Medicine, Sun Yat-sen University, Guangzhou, People's Republic of China.
  16. Yingle Liu: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
  17. Yu Zhou: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
  18. Ke Lan: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.
  19. Yu Chen: State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China. ORCID
PMID: 32228226 DOI: 10.1080/22221751.2020.1747363

Abstract

Circulating in China and 158 other countries and areas, the ongoing COVID-19 outbreak has caused devastating mortality and posed a great threat to public health. However, efforts to identify effectively supportive therapeutic drugs and treatments has been hampered by our limited understanding of host immune response for this fatal disease. To characterize the transcriptional signatures of host inflammatory response to SARS-CoV-2 (HCoV-19) infection, we carried out transcriptome sequencing of the RNAs isolated from the bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) specimens of COVID-19 patients. Our results reveal distinct host inflammatory cytokine profiles to SARS-CoV-2 infection in patients, and highlight the association between COVID-19 pathogenesis and excessive cytokine release such as CCL2/MCP-1, CXCL10/IP-10, CCL3/MIP-1A, and CCL4/MIP1B. Furthermore, SARS-CoV-2 induced activation of apoptosis and P53 signalling pathway in lymphocytes may be the cause of patients' lymphopenia. The transcriptome dataset of COVID-19 patients would be a valuable resource for clinical guidance on anti-inflammatory medication and understanding the molecular mechansims of host response.

Keywords

COVID-19 cytokine inflammation lymphopenia transcriptome profiling

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MeSH Term

Apoptosis
Betacoronavirus
Bronchoalveolar Lavage Fluid
COVID-19
Chemokines
Coronavirus Infections
Cytokines
Humans
Leukocytes, Mononuclear
Lymphopenia
Pandemics
Pneumonia, Viral
RNA-Seq
SARS-CoV-2
Signal Transduction
Transcriptome
Tumor Suppressor Protein p53

Chemicals

Chemokines
Cytokines
TP53 protein, human
Tumor Suppressor Protein p53
Journal Article

Links to CNCB-NGDC Resources

BioProject: PRJCA002326 (Transcriptome of patients infected with SARS-CoV-2)
GSA: CRA002390 (Transcriptome of patients infected with SARS-CoV-2)
GEN: GEND000183 (Transcriptome of patients infected with SARS-CoV-2)

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