AB462324

Annotated Information

Function

The recently cloned blast resistance (R) gene Pi-km protects rice crops against specific races of the fungal pathogen Magnaporthe oryzae in a gene-for-gene manner.[1]High-resolution genetic mapping and sequencing of the gene region in the Pikm-containing cultivar Tsuyuake narrowed down the candidate region to a 131-kb genomic interval[2]

常规信息

Pik-m 是稻瘟病抗性位点Pik 上的一个主效抗病等位基因，供体是Tsuyuake

基因的发现与命名

Kiyosawa 报道Minehikari 中含有一个与Pi-k 连锁的抗性基因Pi-m，但后来多个试验表明Pi-m 与Pi-k 等位，于是将Pi-m 重新命名为Pi-km (Kiyosawa, 1978)。

基因的定位

Pi-km 初步定位于水稻第11 染色体长臂近末端区域，SSR 标记RM254 和RM144 之间，遗传距离分别是13.4cM 和1.2cM；通过生物信息学分析，发展新的分子标记，最终精细定位在BAC克隆OSJNBa0036K13 内的84kb区间内(Li et al., 2007)。

基因克隆与生物学功能分析

序列分析和遗传互补实验表明，Pi-km 是由两个紧密连锁的具有独立功能NBS-LRR类基因(Pikm1-TS 和Pikm2-TS)组成。其中，Pikm1-TS 包含有62bp 5'-UTR、3432bp 编码区、2个内含子(119bp, 2769bp)和190bp 3'-UTR；Pikm2-TS 与Pikm1-TS 不同源，包含109bp 5'-UTR、3066bp 编码区、1个内含子(163bp)和283bp 3'-UTR(Ashikawa et al., 2008)。 Pikm1-TS 和Pikm2-TS 的编码产物均是NBS-LRR类抗病蛋白，长度分别为1143aa和1021aa。他们在结构上有些小的差异：Pikm1-TS 氨基端发现有nT基序，属于CC结构，而Pikm2-TS 虽也发现有nT基序，但未发现有CC结构；Pikm1-TS 羧基端有非LRR结构，而Pikm2-TS 没有(Ashikawa et al., 2008)。

备注

NBS-LRR 类抗病蛋白

Labs working on this gene

Please input related labs here.

References

1. Stefano Costanzo;Yulin Jia,Sequence variation at the rice blast resistance gene Pi-km locus: Implications for the development of allele specific markers.Plant Science, 2010, 178(6): 523-530

2.Ikuo Ashikawa;Nagao Hayashi;Hiroko Yamane;Hiroyuki Kanamori;Jianzhong Wu;Takashi Matsumoto;Kazuko Ono;Masahiro Yano, Two Adjacent Nucleotide-Binding Site–Leucine-Rich Repeat Class Genes Are Required to Confer Pikm-Specific Rice Blast Resistance.Genetics, 2008, 180(4): 2267-2276

3. Luo-Ye Li;Ling Wang;Jin-Xue Jing;Zhen-Qi Li;Fei Lin;Li-Fei Huang;Qing-Hua Pan,The Pikm gene, conferring stable resistance to isolates of Magnaporthe oryzae, was finely mapped in a crossover-cold region on rice chromosome 11. Molecular Breeding, 2007, 20(2): 179-188

4. Shigehisa Kiyosawa,Identification of Blast-Resistance Genes in Some Rice Varieties.Japanese Journal of Breeding, 1978, 28(4): 287-296

Structured Information

Gene Name	AB462325
Description	3458 bp mRNA linear PLN 20-MAR-2009.
Version	AB462325.1 GI:207367331
Length	3458 bp
Definition	Oryza sativa Japonica Group Pikm2-TS mRNA for NBS-LRR class disease resistance protein, complete cds.
Source	Oryza sativa Japonica Group (Japanese rice) ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 11
Location	Chromosome11:110..3175
Sequence Coding Region	110..3175
Expression	GEO Profiles:AB462325
Genome Context	{{{GCID}}}
Gene Structure	{{{GSID}}}
Coding Sequence	{{{CDNA}}}
Protein Sequence	<aaseq>MELVVGASEATMKSLLGKLGNLLAQEYALISGIRGDIQYINDEL ASMQAFLRDLSNVPEGHSHGHRMKDWMKQIRDIAYDVEDCIDDFAHRLPQDSISDAKW SFLLTKIYELWTWWPRRVIASNIAQLKVRAQQIADRRSRYGVNNPEHLDSSSSARTRA VNYEIAEYQVTSPQIIGIKEPVGMKTVMEELEVWLTNPQAENGQAVLSIVGFGGVGKT TIATALYRKVSEKFQCRASVAVSQNYDQGKVLNSILSQVSNQEQGSSTTISEKKNLTS GAKSMLKTALSLLRGNCICQPENDGNPDNTPIRLQETTDDDQNPRKLEQLLAEKSYIL LIDDIWSAETWESIRSILPKNNKGGRIIVTTRFQAVGSTCSPLETDRLHTVDFLTDDE SQNLFNTSICESKIRKDSNKVDEQVPEEIWKICGGLPLAIVSMAGLVACNPRKACCDW SKLCKSLFPEQETPLTLDGVTRILDCCYNDLPADLKTCLLYLSIFPKGWKISRKRLSR RWIAEGFANEKQGLTQERVAEAYFNQLTRRNLVRPMEHGSNGKVKTFQVHDMVLEYIM SKSIEENFITVVGGHWQMTAPSNKVRRLSMQSSGSNRGSSTKGLNLAQVRSLTVFGNL NHVPFHSFNYGIIQVLDLEDWKGLKERHMTEICQMLLLKYLSIRRTEISKIPSKIQKL EYLETLDIRETYVRDLPKSIVQLKRIISILGGNKNTRKGLRLPQEKSKKPIKNPSPQG KTKEPAKKGFLSQEKGKGAMKALRVLSGIEIVEESSEVAAGLHQLTGLRKLAIYKLNI TKGGDTFKQLQSSIEYLGSCGLQTLAINDENSEFINSLGDMPAPPRYLVALELSGKLE KLPKWITSITTLNKLTISVTVLRTETLEILHILPSLFSLTFAFSLSAAKQDQDIIKDI LENNKLDSDGEIVIPAEGFKSLKLLRFFAPLVPKLSFLDKNAMPALEIIEMRFKDFEG LFGIEILENLREVHLKVSDGAEAITKFLVNDLKVNTEKPKVFVDGIVTA</aaseq>
Gene Sequence	<dnaseqindica>1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441..1501#1561..1621#1681..1741#1801..1861#1921..1981#2041..2101#2161..2221#2281..2341# 2401..2461#2521..2581#2641..2701#2761..2821#2881..2941#3001..3061#3121..3181#3241..3301#3361..3421# aggacacgga aattctcagc aggttcgcgc ttgatctgaa ctgtctgtgg ctcatactct cttgtgcttg caacagctga aagttgcagt gagaagtaca gagaacaaga tggagttggt ggtaggtgct tccgaagcca ccatgaaatc tctcttgggc aagctgggca atcttctagc ccaggagtat gctctcatca gcggtatccg tggtgacatc cagtacatca atgacgagct tgccagcatg caggccttcc tccgtgatct cagcaacgtg ccagagggtc acagtcatgg ccaccggatg aaggactgga tgaagcagat ccgagacatc gcctatgatg ttgaggactg tatcgatgac tttgcccacc gcctccctca ggattccatc agcgatgcca aatggtcctt cctactcaca aaaatctatg aactatggac atggtggcca cgtcgtgtga ttgcttccaa cattgcccaa ctcaaggtac gggcacaaca gatcgcagat cgacgtagta gatacggagt gaacaaccca gaacaccttg acagtagcag cagtgccagg acccgtgctg tcaattacga aattgctgag tatcaggtca caagccctca gatcattggt ataaaggagc ctgtggggat gaagacggtc atggaggagc ttgaggtttg gttaactaat cctcaagctg aaaatgggca agctgttctg tccatagtcg gttttggagg tgtgggaaag actaccattg ccacagcatt gtacagaaaa gtcagtgaaa aatttcagtg ccgggcatca gtagctgtgt ctcagaacta tgaccaaggc aaagtcctca atagtattct gagtcaagtc agcaatcagg agcagggcag cagcacaaca attagtgaga aaaagaacct cacctcaggc gctaagagca tgttgaagac agccctgtca ctgctcagag gtaattgtat atgtcagcca gaaaatgatg gaaaccctga taatacacca atcaggctgc aggaaacaac ggacgatgat caaaacccca gaaaactgga acagctcctg gccgaaaaga gttatatcct cttgattgat gacatttggt ctgccgaaac atgggagagt atcagatcga ttttgcctaa aaataataaa ggcggtagaa taatagtgac tacaagattt caagctgttg gttcaacatg ctcccctctt gaaactgatc gtttgcatac agttgatttt ctcaccgatg acgagtccca aaacttattc aatacaagta tttgtgaatc aaagataaga aaagatagca acaaagtaga cgagcaagtc cctgaggaaa tatggaaaat atgtggggga ttgcctttgg ccatagtcag catggctggt cttgtcgcct gcaacccaag gaaagcctgc tgcgattgga gtaaactttg caaatcatta tttccagagc aagaaactcc tcttaccctc gatggtgtta caaggatact ggattgttgt tacaatgatt tgcctgcgga tctgaagact tgcttattgt acttgagtat atttccgaag ggttggaaaa ttagtaggaa acgtttgtcc cggcgatgga tagctgaagg ttttgctaat gagaagcaag ggttaaccca ggaaagagtt gcagaggcat actttaatca actcacaaga aggaacttag tacgtcccat ggagcatggc agcaatggga aggtaaaaac gtttcaagtt catgacatgg ttcttgaata catcatgtcc aaatcaatcg aagagaattt tattactgtg gttggtggac actggcagat gactgcacca agcaataaag tccgtcgact gtcgatgcaa agcagtggat ccaatcgtgg aagttcaaca aaaggcctga acttggctca agtgagatca ctgacggtgt ttgggaacct gaaccatgtg ccattccatt cattcaacta tgggataata caggtgctgg atcttgagga ctggaagggt ttgaaagaga gacatatgac ggagatatgt caaatgcttt tactcaagta tttgagcatc cgacgaacag aaatttccaa aattccctcc aagattcaga aacttgagta cttggaaact cttgacataa gggagacata tgtcagggac ctgcctaagt caatagtcca gctaaaacgg atcattagca tacttggagg gaataaaaac acacggaagg ggctgaggtt gcctcaagaa aaaagtaaga agccaattaa aaacccgtcg cctcaaggaa aaacaaagga gcccgcaaag aaaggattct tatcccaaga aaaaggtaaa ggcgcaatga aagcactccg tgtactgtca gggattgaga ttgttgagga atcatcagaa gtagctgcag gccttcatca gttgacaggg ctaaggaagc ttgccatata caagctcaat ataacaaagg gtggtgatac cttcaaacaa ttacagtcct ccattgagta ccttggcagc tgtggtctgc agactctggc catcaatgat gagaattctg aatttatcaa ctcactgggc gacatgcccg cgcctccaag atatcttgtc gcccttgagc tgtctggcaa gttggagaag ctacccaagt ggatcaccag catcactact ctcaacaagc taaccatatc tgtaacagtt cttaggactg aaactttgga gatcctccac attttacctt cattgttttc cctcaccttc gccttttcac ttagtgcagc gaagcaggat caggacataa taaaggacat ccttgagaat aataaattgg acagtgatgg ggaaatcgtc attccagctg aaggattcaa gagtcttaag ctgcttcgct tctttgcacc tttagtgccg aagctcagct ttttggacaa gaatgcaatg ccagcactcg aaatcattga aatgcggttt aaagacttcg aaggtctatt tggcatcgaa atccttgaaa atctccgtga ggtgcatctc aaagttagtg atggggcaga agcaataacc aagttccttg taaatgattt gaaggttaat actgagaaac caaaagtatt tgttgatggc atcgtcactg catgagaagt aaaattgctg caaatcggag aacttaccaa tcatctgagg cttcccctct attattactc tcttagaata tattgttatt attgctcacc ttgcaaaata aaatagggat ggcatagcat attgctacaa cgtaccatgg ttccatcata gttgatttca cttgtcatta cagtgtctgt tcagttgtgt tttctattaa taaaagggag atctccgcaa gaaaccatta ttatacttat attcggttat tgaactctat aaatgatggg attgctat</dnaseqindica>
External Link(s)	NCBI Gene:AB462325, [1]