LOC Os08g30910

Introduction：

Histone lysine methylation plays an important role in heterochromatin formation and reprogramming of gene expression. SET-domain-containing proteins are shown to have histone lysine methyltransferase activities. A large number of SET-domain genes are identified in plant genomes. The function of most SET-domain genes is not known. In this work, we studied the 12 rice (Oryza sativa) homologs of Su(var)3–9, the histone H3 lysine 9 (H3K9) methyltransferase identified in Drosophila. Several rice SUVHs (i.e. SDG714, SDG727, and SDG710) were found to have an antagonistic function to the histone H3K9 demethylase JMJ706, as down-regulation of these genes could partially complement the jmj706 phenotype and reduced histone H3K9 methylation. Down-regulation of a rice Su(var)3–9 homolog (SUVH), namely SDG728, decreased H3K9 methylation and altered seed morphology. Overexpression of the gene increased H3K9 methylation. SDG728 and other SUVH genes were found to be involved in the repression of retrotransposons such as Tos17 and a Ty1-copia element. Analysis of histone methylation suggested that SDG728-mediated H3K9 methylation may play an important role in retrotransposon repression.

Gene Clone Chr Locn Hit Dir FromTo Type ______________________________________________________________________________________________

Os08g30910 PFG_3B-00273.L chr08 W/19082792-19086784 1000-Promotor C 19081650-19081877 PFG T-DNA Os08g30910 GCAGAGCTGTACCGTGC chr08 W/19082792-19086784 1000-Promotor W 19081897-19081913 smRNA MPSS Os08g30910 CTGTACCGTGCAGGCAC chr08 W/19082792-19086784 1000-Promotor W 19081903-19081919 smRNA MPSS Os08g30910 smRNA90883_1x chr08 W/19082792-19086784 1000-Promotor W 19082129-19082152 smRNA CSRDB Os08g30910 AGAAATGGGCTCGAGTT chr08 W/19082792-19086784 1000-Promotor C 19082140-19082156 smRNA MPSS Os08g30910 CGGCTCGAGTTGGTGCC chr08 W/19082792-19086784 1000-Promotor W 19082190-19082206 smRNA MPSS Os08g30910 FL039956 chr08 W/19082792-19086784 Exon C 19085021-19085620 RTIM Tos17 Os08g30910 RMD_TTosR-04Z11HS51_TosRS chr08 W/19082792-19086784 Exon C 19085209-19085359 RMD T-DNA Os08g30910 RMD_TTosR-04Z11HT27_TosRS chr08 W/19082792-19086784 Exon C 19085216-19085790 RMD T-DNA Os08g30910 PFG_4A-00775.R chr08 W/19082792-19086784 Exon W 19085272-19086009 PFG T-DNA Os08g30910 RMD_04Z11AA68 chr08 W/19082792-19086784 Exon W 19085355-19085620 RMD T-DNA Os08g30910 RMD_TTosL-04Z11AA68_TosRS chr08 W/19082792-19086784 Exon W 19085355-19085620 RMD T-DNA Os08g30910 BT068011 chr08 W/19082792-19086784 Exon W 19085819-19086838 Maize cDNA Os08g30910 FL047512 chr08 W/19082792-19086784 Exon C 19086009-19086608 RTIM Tos17 Os08g30910 FL047532 chr08 W/19082792-19086784 Exon C 19086009-19086608 RTIM Tos17 Os08g30910 FL051969 chr08 W/19082792-19086784 Exon C 19086009-19086608 RTIM Tos17 Os08g30910 NC0178_0_103_1A chr08 W/19082792-19086784 Exon C 19086009-19086521 RTIM Tos17 Os08g30910 BT055122 chr08 W/19082792-19086784 Exon W 19086071-19086832 Maize cDNA Os08g30910 FL012413 chr08 W/19082792-19086784 Exon W 19086112-19086502 RTIM Tos17 Os08g30910 FL049049 chr08 W/19082792-19086784 Exon W 19086151-19086735 RTIM Tos17 Os08g30910 NE9001_0_502_1A chr08 W/19082792-19086784 Exon C 19086155-19086411 RTIM Tos17 Os08g30910 BT055367 chr08 W/19082792-19086784 Exon W 19086176-19086638 Maize cDNA Os08g30910 BT068432 chr08 W/19082792-19086784 Exon W 19086176-19086638 Maize cDNA Os08g30910 AY187719 chr08 W/19082792-19086784 Exon W 19086185-19086562 Maize cDNA Os08g30910 At2g23740 chr08 W/19082792-19086784 Exon W 19086185-19086564 Arabidopsis cDNA Os08g30910 At5g43990 chr08 W/19082792-19086784 Exon W 19086185-19086638 Arabidopsis cDNA Os08g30910 EU970110 chr08 W/19082792-19086784 Exon W 19086191-19086537 Maize cDNA Os08g30910 At3g03750 chr08 W/19082792-19086784 Exon W 19086191-19086537 Arabidopsis cDNA Os08g30910 FL049037 chr08 W/19082792-19086784 300-UTR3 C 19086206-19086805 RTIM Tos17 Os08g30910 EU968739 chr08 W/19082792-19086784 Exon W 19086317-19086560 Maize cDNA Os08g30910 FL048039 chr08 W/19082792-19086784 Exon W 19086347-19086946 RTIM Tos17 Os08g30910 FL048371 chr08 W/19082792-19086784 Exon W 19086347-19086946 RTIM Tos17 Os08g30910 FL048640 chr08 W/19082792-19086784 Exon W 19086347-19086946 RTIM Tos17 Os08g30910 T36759T chr08 W/19082792-19086784 Exon W 19086347-19086798 RTIM Tos17 Os08g30910 T36794T chr08 W/19082792-19086784 Exon W 19086347-19086802 RTIM Tos17 Os08g30910 NE4522_0_502_1A chr08 W/19082792-19086784 300-UTR3 C 19086386-19087072 RTIM Tos17 Os08g30910 FL028481 chr08 W/19082792-19086784 Exon W 19086407-19087006 RTIM Tos17 Os08g30910 NC2294_0_502_1A chr08 W/19082792-19086784 300-UTR3 C 19086528-19087036 RTIM Tos17 Os08g30910 FL039835 chr08 W/19082792-19086784 Exon W 19086674-19087269 RTIM Tos17 Os08g30910 FL039883 chr08 W/19082792-19086784 Exon W 19086674-19087273 RTIM Tos17 Os08g30910 FL040227 chr08 W/19082792-19086784 Exon W 19086674-19087273 RTIM Tos17 Os08g30910 FL040231 chr08 W/19082792-19086784 Exon W 19086674-19087273 RTIM Tos17 Os08g30910 FL040245 chr08 W/19082792-19086784 Exon W 19086674-19087207 RTIM Tos17 Os08g30910 FL040441 chr08 W/19082792-19086784 Exon W 19086674-19086918 RTIM Tos17 Os08g30910 FL040529 chr08 W/19082792-19086784 Exon W 19086674-19087167 RTIM Tos17 Os08g30910 FL040652 chr08 W/19082792-19086784 Exon W 19086674-19087273 RTIM Tos17 Os08g30910 FL040775 chr08 W/19082792-19086784 Exon W 19086674-19087273 RTIM Tos17 Os08g30910 FL041055 chr08 W/19082792-19086784 Exon W 19086674-19087273 RTIM Tos17 Os08g30910 FL041071 chr08 W/19082792-19086784 Exon W 19086674-19087273 RTIM Tos17 Os08g30910 NF6033_0_401_1A chr08 W/19082792-19086784 Exon W 19086674-19087273 RTIM Tos17 Os08g30910 NF6033_0_405_1A chr08 W/19082792-19086784 Exon W 19086674-19087273 RTIM Tos17 Os08g30910 NF6033_0_501_1A chr08 W/19082792-19086784 Exon W 19086674-19087065 RTIM Tos17 Os08g30910 NF6817_0_404_1A chr08 W/19082792-19086784 Exon W 19086674-19087217 RTIM Tos17 Os08g30910 FL019847 chr08 W/19082792-19086784 300-UTR3 W 19086878-19087171 RTIM Tos17 Os08g30910 FL019972 chr08 W/19082792-19086784 300-UTR3 W 19086878-19087477 RTIM Tos17 Os08g30910 FL021193 chr08 W/19082792-19086784 300-UTR3 W 19086878-19087479 RTIM Tos17