Os01g0178300
The rice Os01g0178300 was reported as OsCDT3 in 2013 [1] by researchers from Japan.
Contents
Annotated Information
Gene Symbol
- Os01g0178300 <=> OsCDT3,CDT3
Function
- OsCDT3 encodes a predicted peptide of only 53 amino acid residues rich in cysteine.
- OsCDT3 anchoring to the plasma membrane may play a role in stopping entry of Al into the root cells by binding Al, therefore, contributing to high Al tolerance in rice.
Phenotypic analysis
- Knockdown of this gene resulted in decreased tolerance to Al, but did not affect the tolerance to Cd. The aluminum (Al) content in the root residues including cell wall and the plasma membrane of knockdown lines decreased, but the Al concentration in the root cell sap increased compared with those of the wild-type rice.
Expression
- The expression of OsCDT3 was low in both the roots and shoots in the absence of Al [Figure 3(a)]. However, in the presence of Al, the expression was up-regulated by six times in the roots, but not in the shoots [Figure 3(a)]. Spatial expression analysis showed that OsCDT3 was expressed in both the root tips (0–1 cm) and mature regions (1–2 cm) and the expression in both segments are up-regulated by Al [Figure 3(b)].
- A time-course experiment showed that induction of OsCDT3 occurred at 3 h after the exposure to Al and reached the maximum at 12 h [Figure 3(d)]. Furthermore, the expres- sion of OsCDT3 is specifically induced by Al, but not by other metals including Cd and La, and by low pH [Figure 3(e)].
Subcellular localization
- The subcellular localization of OsCDT3 was examined by transiently expressing GFP-tagged OsCDT3 in onion epidermal cells. One of the rice homologs, OsCDT1, was reported to be localized at the plasma membrane and secreted outside the cells where it associates with the cell wall
- Unlike OsCDT1, OsCDT3 is not secreted out of the cells, but anchored to the plasma membrane.
Figure 6. Subcellular localization of OsCDT3.[1].
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Labs working on this gene
- Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, Japan
References
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