Os01g0185300

Ferulates (FA) can crosslink different arabinoxylan molecules in the cell wall of grasses via diferulate and oligoferulate bridges.

Annotated Information

Function

• Ferulates (FA) can crosslink different arabinoxylan molecules in the cell wall of grasses via diferulate and oligoferulate bridges. The FA content in the cell walls and the transcript levels of rice genes Os05g08640, Os06g39470, Os01g09010 and Os06g39390, are both higher in the stems than in the leaves.
• The members of the PF02458 family may encode for putative arabinoxylan feruloyl transferases (AFT) and be involved in acylating arabinoxylans with FA.

Expression

• No significant differences in transcript levels of the highly expressed genes were detected between pAFT-A transgenic events A5 and A11 or between pAFT-B transgenic events B1 and B11,when the tissues were studied separately or when they were combined in the statistical analyses. Therefore, the following statistical analyses combine the two events examined for each construct, whereas the results for the individual events and tissues are presented in Fig. 3.

'Fig. 3 Transcript levels of genes targeted by a pAFT-A or b pAFT-B constructs in transgenic and control plants in experiment 2. a Transcript levels of genes from group I in the transgenic events A5 and A11 compared with the control. b, c The expression levels of groups III (b), IV (c) genes in the transgenic events B1 and B11 compared with the control. Values in the Y axes are normalized and calibrated values using the formula (1 ? efficiency)-DDCT method(Livak and Schmittgen 2001). All values were divided by 1,000 to simplify the graphs. The same calibrator was used for all genes so values are comparable across genes. Error bars are SE of the means'

Labs working on this gene

• Department of Plant Sciences, University of California,
• Dept. de Agronomı ´a y Mejora Gene ´tica Vegetal,Instituto de Agricultura Sostenible, CSIC,
• Department of Crop Genetics, John Innes Centre,