Os01g0633100
The rice Os01g0633100 was reported as OsAGPL2 in 2014 [1] by researchers from the USA and Japan.
Contents
Annotated Information
Figure 1. Morphology of wild-type, shr1, shr1a and shr2 de-hulled seeds. A cross-section of each seed is also shown..[1].
Gene Symbol
- Os01g0633100 <=> OsAGPase,AGPase
Function
- L2 subunit of cytosolic isoform of ADPglucose pyrophosphorylase (AGPase) encoded by OsAGPL2 is essential for both catalysis and allosteric regulatory properties of the heterotetramer enzyme.
- Missense and nonsense mutations in the OsAGPL2 gene result in similar decreases in seed weight
Phenotypic analysis
- The researchers isolated several allelic missense and nonsense OsAGPL2 mutants by N-methyl-N-nitrosourea (MNU) treatment of fertilized egg cells and by TILLING (Targeting Induced Local Lesions in Genomes).
- Interestingly, seeds from three of the missense mutants (two containing T139I and A171V) were severely shriveled and had seed weight and starch content comparable with the shriveled seeds from OsAGPL2 null mutants.
- Results from kinetic analysis of the purified recombinant enzymes revealed that the catalytic and allosteric regulatory properties of these mutant enzymes were significantly impaired.
- The missense heterotetramer enzymes and the S2b homotetramer had lower specific (catalytic) activities and affinities for the activator 3-phosphoglycerate (3-PGA). The missense heterotetramer enzymes showed more sensitivity to inhibition by the inhibitor inorganic phosphate (Pi) than the wild-type AGPase, while the S2b homotetramer was profoundly tolerant to P i inhibition.
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Labs working on this gene
- Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA
- Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581 Japan
- Department of Genetic Resources Technology, Kyushu University, Fukuoka, 812-8581 Japan
- Kazusa DNA Research Institute, Department of Plant Genome Research, Kisarazu, Japan
- Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, 010-0195 Japan
References
- ↑ 1.0 1.1 Tuncel A, Kawaguchi J, Ihara Y, Matsusaka H, Nishi A, Nakamura T, Kuhara S, Hirakawa H, Nakamura Y, Cakir B, Nagamine A, Okita TW, Hwang SK, Satoh H. The rice endosperm ADP-glucose pyrophosphorylase large subunit is essential for optimal catalysis and allosteric regulation of the heterotetrameric enzyme. Plant Cell Physiol. 2014 Jun;55(6):1169-83. doi: 10.1093/pcp/pcu057. Epub 2014 Apr 18. PubMed PMID: 24747952.
