Os01g0678600
The rice Os01g0678600 was reported as ASL1 in 2013 [1] by researchers from China.
Contents
Annotated Information
Figure 1. Characterization of the asl1 mutants.[1].
Gene Symbol
- Os01g0678600 <=> ASL1,OsASL1
Function
- Plastid ribosomal proteins (PRPs) are essential for ribosome biogenesis, plastid protein biosynthesis, chloroplast differentiation, and early chloroplast development. * In their is study, the researchers identifies the first rice PRP mutant, asl1 (albino seedling lethality1), which exhibits an albino lethal phenotype at the seedling stage.
- ASL1 is essential for chloroplast development in rice.
- ASL1 is involved in the establishment of the transcription/translation apparatus and plastid-to-nucleus signaling.
- ASL1 plays an important role in rice seedlings, especially at early stages.
Phenotypic analysis
- The asl1 mutant is an albino, seedling-lethal mutant isolated from japonica rice Jiahua1 irradiated with 60 Co gamma rays. Although asl1 seeds germinated, all leaves of mutant plants exhibited an albino phenotype (Figure 1A) and the seedlings did not survive past the four- leaf stage (Figure 1B).
- In addition, the contents of Chl a, Chl b, and Car approached zero in asl1 mutant plants (Figure 1C). These results suggested that the albino phenotypes of the young asl1 seedlings are caused by the reduction in total Chl content.
Expression
- The researchers used RT-PCR to examine the expression of ASL1 in WT plants (Figure 5A). The researchers detected a significantly high level of expression in young leaves, culms, and flag leaves but weak expression in roots and young panicles. The ASL1 transcript was substantially reduced in asl1 mutants, indicating that the mutation in ASL1 causes a decreased transcript level.
- Light plays a major role in regulating ASL1 expression.
Subcellular localization
- The ASL1 protein was predicted to localize to chloroplasts by ChloroP and TargetP.
- Subcellular localization analysis of ASL1ASL1 protein is localized to the chloroplast.
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Labs working on this gene
- Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China
- Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
- Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
