Os01g0689451

The rice Os01g0689451 was reported as OsHRZ1 in 2013 ^[1] by researchers from Japan.

Annotated Information

Figure 4. Fe-deficiency tolerance of the HRZ-knockdown plants.^[1].

OsHRZ1 is susceptible to degradation in roots irrespective of iron conditions.
The expression of iron deficiency-inducible genes involved in iron utilization is enhanced in OsHRZ-knockdown plants, mostly under iron-sufficient conditions.
OsHRZ1 is iron-binding sensors that may negatively regulate iron acquisition under conditions of iron sufficiency.
OsHRZ1 is Fe- and Zn-binding ubiquitin ligases.

The researchers generated HRZ-knockdown rice lines by RNA interference (RNAi) using partial sequences of OsHRZ1 or OsHRZ2 (HRZ1i and HRZ2i lines, respectively).
The HRZ2i lines showed reduced OsHRZ2 expression in Fe-sufficient and -deficient roots and leaves compared with the non-transformants (NT), and also moderate reduction in OsHRZ1 and OsHORZ1 expression in Fe-sufficient leaves.
These lines also tended to show reduced expression of OsHRZ1 in Fe-deficient roots. HRZ1i line 1 showed only a tendency of slight reduction in the expression of OsHRZ1 in roots and OsHORZ1 in leaves, but none in OsHRZ2 expression. These knockdown lines grew healthily with no visible phenotype under normal growth conditions.
To analyse the Fe nutrition in these lines, Fe-deficiency treatment was imposed hydroponically. A gradual decrease in leaf chlor-ophyll levels occurred, an indicator of Fe deficiency in plants 2 . The knockdown lines, especially HRZ2i, retained significantly higher levels of leaf chlorophyll than NT under conditions of Fe deficiency.

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Japan Science and Technology Agency, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

↑ ^1.0 ^1.1 Kobayashi T, Nagasaka S, Senoura T, Itai RN, Nakanishi H, Nishizawa NK. Iron-binding haemerythrin RING ubiquitin ligases regulate plant iron responses and accumulation. Nat Commun. 2013;4:2792. doi: 10.1038/ncomms3792. PubMed PMID: 24253678;