Os01g0734466

• Reactive oxygen species(ROS) molecules generated by OsrbohA and OsrbohE regulate different signaling pathways in the plant immune response.

Annotated Information

'Figure 3. Expression levels of OsrbohA (Os-A), OsrbohB (Os-B),OsrbohD (Os-D), and OsrbohE (Os-E) in OsrbohA-RNAi3 (A) and OsrbohE-RNAi2 (B). The amount of each mRNA was calculated from the threshold point located in the log-linear range of RT-PCR. Open bars, control cultured cells; solid bars, each RNAi-transformant cultured cells. Standard samples with known template amounts were used for quantification of each mRNA. The y-axis represents % change relative to the amount of mRNA in control cultured rice cells. The error bars indicate standard deviation of the mean of three experiments.'

Function

• OsrbohA and OsrbohE knockdown lines showed that rapid H2O2 generation is caused by OsrbohA.
• Hypersensitive cell death was decreased only in the OsrbohA knockdown line.
• The researchers further demonstrated that among immune related genes, the induction of EL2 and LOX genes is controlled by ROS generated by OsrbohE, whereas expression of Cht-1 gene is regulated by both OsrbohA and OsrbohE.

Expression

• Real-time RT-PCR using specific primer sets for OsrbohA, OsrbohB, OsrbohD, and OsrbohE showed that mRNA levels of OsrbohA in OsrbohA-RNAi3 were decreased compared to the control cells, whereas no change in OsrbohB, OsrbohD, and OsrbohE expression levels were observed in OsrbohA-RNAi3 (Figure 3A).OsrbohE expression levels were also decreased in OsrbohE-RNAi2; however, mRNA transcripts of the other Osrboh homologs were the same as in control cells(Figure 3B).

Immune related-genes regulated under OsrbohA

• EL2, Cht-1, EREBP1, CCD1, and LOX— were selected and the researchers examined the induction patterns of these genes in OsrbohA-RNAi3 and OsrbohE-RNAi2 cultured rice cells.

Labs working on this gene

• Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), 8916-5, Takayama Ikoma, Nara 630-0101, Japan;
• Department of Environmental Biology, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama Shiga, 526-0829, Japan.

References

• Alvarez ME, Pennell RI, Meijer PJ, Ishikawa A, Dixon RA, Lamb C (1998) Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell 92:773–784