Os01g0853400

The rice Os01g0853400 was reported as COI1 in 2012 by researchers from China.

Annotated Information

Gene Symbol

• Os01g0853400 <=> COI1,OsCOI1a,OsCOI1,Os_F0728

Function

• The jasmonic acid (JA) pathway plays a key role in plant defense responses against herbivorous insects.
• CORONATINE INSENSITIVE1 (COI1) is an F-box protein essential for all jasmonate responses.
• OsCOI1 is an indispensable signaling component, controlling JA-regulated defense against chewing insect (LF) in rice plants, and COI1 is also required for induction of TrypPI, POD and PPO in rice defense response to LF infestation.

Phenotypic analysis

• The researchers silenced OsCOI1 in rice plants via RNA interference (RNAi) to determine the role of OsCOI1 in rice defense against rice leaf folder (LF) Cnaphalocrocis medinalis, a chewing insect, and brown planthopper (BPH) Nilaparvata lugens, a phloem-feeding insect.
• In wild-type rice plants (WT), the transcripts of OsCOI1 were strongly and continuously up-regulated by LF infestation and methyl jasmonate (MeJA) treatment, but not by BPH infestation.
• The abundance of trypsin protease inhibitor (TrypPI), and the enzymatic activities of polyphenol oxidase (PPO) and peroxidase (POD) were enhanced in response to both LF and BPH infestation, but the activity of lipoxygenase (LOX) was only induced by LF.
• The RNAi lines with repressed expression of OsCOI1 showed reduced resistance against LF, but no change against BPH.
• Silencing OsCOI1 did not alter LF-induced LOX activity and JA content, but it led to a reduction in the TrypPI content, POD and PPO activity by 62.3%, 48.5% and 27.2%, respectively.
• In addition, MeJA- induced TrypPI and POD activity were reduced by 57.2% and 48.2% in OsCOI1 RNAi plants.

Expression

• The transcripts of OsCOI1 were strongly and continuously up-regulated by LF infestation and methyl jasmonate (MeJA) treatment, but not by BPH infestation.

Evolution

• The rice genome contains two closely related COI1 genes, OsCOI1a(Os01g0853400) and OsCOI1b(Os05g0449500), which share 83% and 82% sequence identity at the DNA and protein levels, respectively.

Labs working on this gene

• State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Ministry of Agriculture, South China Agricultural University, Wushan, Guangzhou,People’s Republic of China,
• Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, Wushan, Guangzhou, People’s Republic of China
• Plant Protection Institute, Guangdong Academy of Agricultural Sciences of China, Guangzhou, People’s Republic of China,
• College of Life Sciences, South China Agricultural University, Wushan, Guangzhou, People’s Republic of China
• Department of Entomology, Texas A&M University, College Station, Texas, United States of America