Os01g0878700

The rice Os01g0878700 was reported as OsAAP6 in 2014 by researchers from China.

Annotated Information

Gene Symbol

Fig. 2.Expression patterns of OsAAP6 and subcellular localization analysis..

• Os01g0878700 <=> OsAAP6,AAP6

Function

• OsAAP6 greatly enhances root absorption of a range of amino acids and has effects on the distribution of various amino acids.
• OsAAP6 encodes a putative amino acid transporter, which functions as a positive regulator of GPC (grain protein content) in rice.

Expression

• Sixteen tissues from the NILs were assayed for temporal and spatial expression patterns of OsAAP6 by quantitative reverse transcription–PCR (Fig. 2a).
• OsAAP6 transcripts were detected in all examined tissues and were most abundant in the endosperms at 5–12 days after flowering (DAF) (Fig. 2a), the same timeframe as reported for transcripts of OsAAP6 (ref. 23), and there were significantly higher transcript levels in the endosperms of NIL(ZS97) than in endosperm of NIL(NYZ) (Fig. 2a).

Evolution

• To investigate the subcellular localization of OsAAP6 protein, the researchers transiently co-expressed OsAAP6 fused to green fluorescent protein (GFP) with various subcellular markers in rice protoplasts 28–31.
• Co-expression of OsAAP6-GFP and SCAMP1-RFP clearly showed that OsAAP6 was not localized at the plasma membrane or on the trans-Golgi network (Fig. 2n).
• However, OsAAP6-GFP and the endoplasmic reticulum (ER) marker BiP-RFP overlapped completely when co-expressed in rice protoplasts (Fig. 2o). Therefore, OsAAP6 is localized on the ER, but not on the plasma membrane or in the trans-Golgi network.

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Labs working on this gene

• National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
• Shanghai Agrobiological Gene Center, Shanghai 201106, China.