Os01g0907400
The rice Os01g0907400 was reported as JMJ705 in 2013 [1] by researchers from China and France.
Contents
Annotated Information
Figure 2. JMJ705 Overexpression Produces a Leaf Lesion–Mimic Phenotype at the Mature Stage..[1].
Gene Symbol
- Os01g0907400 <=> JMJ705,OsJMJ705
Function
- JMJ705 encodes a histone lysine demethylase that specifically reverses H3K27me2/3.
- Overexpression of JMJ705 reduces the resting level of H3K27me2/3 resulting in preferential activation of H3K27me3-marked biotic stress responsive genes and enhances rice resistance to the bacterial blight disease pathogen Xanthomonas oryzae pathovar oryzae.
- Mutation of JMJ705 reduces plant resistance to the pathogen.
- JMJ705 is involved in methyl jasmonate–induced dynamic removal of H3K27me3 and gene activation.
- The results suggest that JMJ705 is a biotic stress-responsive H3K27me2/3 demethylase that may remove H3K27me3 from marked defense-related genes and increase their basal and induced expression during pathogen infection.
Phenotypic analysis
- To study whether increased expression of JMJ705 could reduce the resting level of H3K27me3 and affect gene expression and plant growth, we produced transgenic rice plants overexpressing JMJ705-FLAG under the strong maize ubiquitin promoter in the Zhonghua11 (ZH11) background (Figure 2A).
- Protein gel blot analysis indicated that the overexpression of the gene resulted in a reduction of the overall H3K27me2/3 levels in the transgenic plants compared with wild type (Figure 1D). The overexpression transgenic lines displayed a leaf lesion-mimic phenotype at the mature stage (Figures 2A and 2B).
Expression
- RT-PCR analysis indicated that JMJ705 was expressed in all tested tissues/organs, with a relatively higher level in rice leaves, and was found to be induced by NaCl, abscisic acid, ethylene (ACC), and JA (see Supplemental Figure 2 online), suggesting that JMJ705 might be involved in stress responses.
- The expression of JMJ705 is induced by stress signals and during pathogen infection.
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Labs working on this gene
- National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China
- Institut de Biologie des Plantes Unité Mixte de Recherche 8618 Université Paris-sud 11, 91405 Orsay, France
