Os01g0927000

• The rice Os01g0927000 was reported as SDG714 in 2007 by researchers from the USA.

Annotated Information

'Figure 2. Methyltransferase Activity and Site Specificity of SDG714 in Vitro.(A) Methyltransferase activity of SDG714. GST and GST-SDG714 fusion proteins were tested for their ability to methylate oligonucleosomes or core histones. Enzymes and substrates used are indicated at top. The left panel shows the Coomassie blue–stained gel. The positions of GST and GSTSDG714 fusion proteins and different histones are indicated at right. Autoradiography in the right panel indicates methyltransferase activity and specificity.(B) and (C) Site specificity of SDG714 (B) and the C-terminal part with pre-SET, SET, and post-SET domains of SDG714 (SDG714C; 319 to 663 amino acids) (C). Methyltransferase assays were performed using substrates, as GST-NH3 contains the N-terminal 1 to 57 amino acids of histone H3 fused with GST and point mutations at histone H3K4, H3K9, or H3K27 to Arg (R), named GST-H3N1-57R4, GST-H3N1-57R9, and GST-H3N1-57R27 respectively. Substrates used are indicated at top. The top panels show Coomassie blue–stained gels, and the positions of GST and GST-SDG714C fusion proteins are indicated at right. Autoradiography in the bottom panels indicates methyltransferase activity and specificity'

Gene Symbol

• Os01g0927000 <=> SDG714

Function

• rice SDG714 (for SET Domain Group Protein714) encodes a histone H3K9-specific methyltransferase.
• histone H3K9 methylation mediated by SDG714 is involved in DNA methylation, the transposition of transposable elements, and genome stability in rice.

Mutation

• Loss-of-function mutants of SDG714 (SDG714IR transformants) generated by RNA interference display a mostly glabrous phenotype as a result of the lack of macro trichomes in glumes, leaves, and culms compared with control plants. These mutants also show decreased levels of CpG and CNG cytosine methylation as well as H3K9 methylation at the Tos17 locus, a copia-like retrotransposon widely used for the generation of rice mutants. Most interestingly, loss of function of SDG714 can enhance transcription and cause the transposition of Tos17.

Labs working on this gene

• State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
• Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
• High-Tech Research Center, Shandong Academy of Agricultural Sciences, Key Laboratory for Genetic Improvement of Crop, Animal, and Poultry of Shandong Province, Ji’nan 250100, China
• College of Life Sciences, Shandong Normal University, Ji’nan 250014, China
• State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China