Os02g0121700
The rice Os02g0121700 was reported as OsLIS in 2013 [1] by researchers from Japan
Contents
Annotated Information
Gene Symbol
- Os02g0121700 <=> OsLIS,LIS
Figure 2. Subcellular localization of linalool synthase.[1].
Function
- Expression of linalool synthase was up-regulated by JA. Vapour treatment with linalool induced resistance to Xoo, and transgenic rice plants overexpressing linalool synthase were more resistance to Xoo, presumably due to the up-regulation of defence-related genes in the absence of any treatment.
- Linalool which is regulated by (OsLIS) plays an important role in JA-induced resistance to Xoo in rice.
Phenotypic analysis
- There were no morphological differences between the transgenic rice plants and the wild type (WT) under normal growth conditions.
- Firstly, to confirm whether overexpressed OsLIS work actively in these transgenic rice plants, the researchers measured the level of linalool by semi-quantitative analysis with GC. In wild-type cv. Nipponbare, linalool was detected without JA treatment. However, the levels of linalool in OsLIS-overexpressing lines significantly accumulate higher than that in WT. The levels of linalool were almost same between transgenic lines, although the expression levels of OsLIS were different between transgenic lines (Fig. 5a).
- This result suggests that the amounts of linalool accumulating in these transgenic lines are maximum levels in rice and the expression level of OsLIS in line 33 is sufficient for accumulation of linalool at maximum in rice.
- When these rice plants were inoculated with Xoo, mean blight lesion length on OsLIS-overexpressing rice plants were consistent across transgenic lines, but significantly shorter than in control plants.
Subcellular localization
- Based on the GFP fusion protein, OsLIS was localized to the chloroplast of transformed cells, indicating that linalool is produced within chloroplasts
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Labs working on this gene
- Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan
References
- ↑ 1.0 1.1 Taniguchi S, Hosokawa-Shinonaga Y, Tamaoki D, Yamada S, Akimitsu K, Gomi K. Jasmonate induction of the monoterpene linalool confers resistance to rice bacterial blight and its biosynthesis is regulated by JAZ protein in rice. Plant Cell Environ. 2014 Feb;37(2):451-61. doi: 10.1111/pce.12169. Epub 2013 Aug 19. PubMed PMID: 23889289.
