Os02g0527600

The rice Os02g0527600 was reported as OsCTR2 in 2013 by researchers from China.

Annotated Information

Figure 5. Subcellular localization of GFP–OsCTR2. Fluorescence of GFP–OsCTR2 (green) and ER-rk (red) in tobacco (A, B) and onion epidermal (C, D) cells. The cell for the co-localization study is indicated with an arrowhead in (A) and (B)..

Gene Symbol

• Os02g0527600 <=> OsCTR2,CTR2

Function

• The osctr2 loss-of-function mutation and expression of the 35S:OsCTR2 1–513 transgene that encodes the OsCTR2 N terminus (residues 1–513) revealed several and many aspects, respectively, of ethylene-induced growth alteration in rice.
• The rice OsCTR2 is closely related to CTR1 and negatively regulates ethylene signalling.

Phenotypic analysis

• To evaluate whether OsCTR1 and OsCTR2 have any roles in ethylene-receptor signalling, the researchers ectopically expressed each gene in a ctr1-1 loss-of-function mutant for complementation testing.
• The expression of OsCTR2 partially rescued the ctr1-1 ethylene response phenotype. Light-grown ctr1-1 seedlings produced small, compact cotyledons and a short root; consistently, ctr1-1 transgenic lines expressing CTR1p:OsCTR2 produced larger cotyledons and a longer root than ctr1-1 seedlings (Fig. 2E).
• At the adult stage, expression of the CTR1p:OsCTR2 transgene largely rescued the ctr1-1 growth inhibition phenotype, and the transgenic lines produced a normal rosette, as in wild-type plants (Col-0)

Subcellular Localization

• Researchers have already studied the subcellular localization of OsCTR2 with GFP-fused OsCTR2.
• Green (GFP–OsCTR2) and red (ER-rk) fluorescence was observed mainly surrounding the nucleus, at the cell edge of tobacco cells, with an uneven, discrete pattern (Fig. 5A), and the ER network was also observed (Fig. 5B).
• To obtain other evidence supporting localization of OsCTR2 at the ER, GFP–OsCTR2 was transiently co-expressed with ER-rk in onion epidermal cells by particle bombardment. Consistently, the fluorescence of GFP–OsCTR2 and ER-rk co-localized (Fig. 5C). The nucleus was surrounded by GFP–OsCTR2 and ER-rk (Fig. 5D), which is consistent with the close association of the ER with the outer membrane of the nuclear envelope.

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Labs working on this gene

• National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China