Os03g0140400
The rice Os03g0140400 was reported as CYP96B4 in 2014 [1] by researchers from China.
Contents
Annotated Information
Figure 4. Subcellular localization of pJIT163_hGFP::SD37 using ER-mCherry and Golgi-mCherry in rice protoplast cells.[1].
Gene Symbol
- Os03g0140400 <=> CYP96B4,OsCYP96B4,SD37,OsSD37
Function
- Slight over-expression of CYP96B4 in transgenic lines promotes plant growth, and RNA interference targeting CYP96B4 in transgenic lines mimics the mutant phenotype
- CYP96B4 may be an important regulator of plant growth that affects plant height in rice.
Phenotypic analysis
- The researchers identified a spontaneous rice dwarf mutant in the indica cultivar 3037 (Oryza sativa L ssp. indica cv. 3037). This mutant displayed a dwarf phenotype during all stages of development, from seedling to grain filling (Figure 1A, 1B).
- All internodes of the sd37 mutant were shorter than those of the wild type (Figure 1C). At the heading stage, the mutant showed a 25–35% reduction in plant height compared to wild-type plants. We thus named this mutant semi-dwarf 37 (sd37). Furthermore, the sd37 mutant had smaller panicles and shorter rachises than the wild type (Figure 1D).
- The grains of sd37 were shorter and wider than those of the wild type (Figure 1E; Table 1). Morphological measurements of the wild type and the sd37 mutant are shown in Table 1. In contrast, the root length of the mutant was equivalent to that of the wild type in young seedlings (Figure 1F).
Expression
- CYP96B4 was predominantly expressed in the shoot apical meristem and developing leaf and root maturation zone but not in the root apical meristem.
Subcellular Localization
- Subcellular Localization analysis of CYP96B4 showed that its protein was ER-localized protein.
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Labs working on this gene
- State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
- National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing, China
References
