Os03g0142800

The rice Os03g0142800 was reported as OsMRP5 in 2014 by researchers from China and Switzerland.

Annotated Information

Table 2 Contents of TP, inorganic P (Pi) and phytic acid P (PA-P) of homozygous transgenic lines (T) and their null siblings (N)..

Gene Symbol

• Os03g0142800 <=> OsMRP5,MRP5

Function

• Seed-specific silencing of OsMRP5 reduces seed phytic acid and weight in rice
• The silencing of the OsMRP5 gene increased the inorganic P (Pi) levels (up to 7.5 times) in amounts more than the reduction of PA-P in brown rice.

Phenotypic analysis

• To analyze the effect of gene silencing on seed P level and composition, the six independent homozygous transgenic lines were assessed for their TP, Pi, and PA-P content, together with their null siblings and Nipponbare (Table 2).
• Significant differences were observed for the contents of Pi, PA-P, and TP in seeds of Nipponbare produced in Hangzhou and Hainan, indicating the existence of environmental effects on seed P content (Table 2). All transgenic lines had substantially and significantly higher levels of Pi and lower levels of PA-P compared to their respective null siblings, which had levels similar to Nipponbare (Table 2).
• The results indicated that seed PA biosynthesis was effectively reduced in transgenic lines,irrespective of growing environments. The TP levelsof three out of five lines tested were significantly less than that of their respective null siblings, while two other lines showed no significant differences (Table 2).
• Nipponbare and null lines had Pi levels less than 0.49 mg/g (#06N), while all transgenic lines had Pi contents ranging from 1.79 to 2.54 mg/g, which amount to 4.9–7.5 times of their respective null siblings (Table 2). Nipponbare seeds produced in Hangzhou had PA-P content of 1.77 mg/g, which was much less than those produced in Hainan (Table 2).
• For the seeds grown in the same site, null lines had PA-P contents similar to that of Nipponbare (Table 2). In sharp contrast, all transgenic lines had PA-P levels substantially lower than their respective null siblings, with reduction rates ranging from 35.8 to 71.9 (Table 2).
• Furthermore, the increase of Pi was more than the reduction of PA-P in transgenic lines, and consequently the proportion of (Pi ? PA-P)/TP was increased (Table 2). These results suggest a potential reduced incorporation of P into other compounds, such as DNA and lipids, to the increase in free Pi content.

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Labs working on this gene

• State Key Laboratory of Rice Biology and Key Laboratory of Nuclear-Agricultural Sciences of the Ministry of Agriculture and Zhejiang Province, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China
• Wuxi Qiushi Agri-Biological Research Center, Wuxi 214105, Jiangsu, China
• Département de Biologie Moléculaire Végétale, Biophore, Université de Lausanne, 1015 Lausanne, Switzerland