Os03g0151800

The rice Os03g0151800 was reported as OsCDC48 in 2015 by researchers from China.

Annotated Information

Function

• The results indicated that the impaired function of OsCDC48 was responsible for the premature senescence and death phenotype.

Phenotypic analysis

• The premature senescence and death 128 (originally coded as HM128) mutant was obtained from mutagenesis of cultivar IR64 using ethyl methane sulfonate (EMS).
• psd128 displayed lower germination rate and slower germination (Figure 1A), retarded growth and development of roots and leaves than those of the wild-type IR64. The most significant changes between psd128 and IR64 appeared at the 6-leaf seedling stage under field conditions when the second leaf of psd128 became yellowish with brown spots.
• This phenotype first appeared on the leaf tip and spread gradually downward to cover the whole leaf blade. Subsequently, the older leaves turn white and wilted gradually, and the plants died almost completely except a few flag leaves at the early heading stage.

Expression

• OsCDC48 was expressed constitutively in the root, stem, leaf and panicle.
• To determine the relative abundance of the transcripts, we performed quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and found that the expression level of OsCDC48 in the roots was significantly higher than those in the stems, leaves and panicles.
• The expression levels of OsCDC48 were similar in the stems, leaves and panicles. Finally, we carried out the promoter assay in transgenic Nipponbare plants and found that GUS was detected in the roots, stems, leaves and panicles/spikelets in consistent to the qRT-PCR.

Subcellular localization

• Subcellular analysis indicated that OsCDC48:YFP fusion proteins were located both in the cytoplasm and nucleus.

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Labs working on this gene

• State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
• Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
• State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
• State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
• State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.