Os03g0237200

The rice Os03g0237200 was reported as LPA1 in 2015 by researchers from Korea and Japan.

Annotated Information

Function

• Loose Plant Architecture1 (LPA1) determines lamina joint bending by suppressing auxin signalling that interacts with C-22-hydroxylated and 6-deoxo brassinosteroids in rice.

Phenotypic analysis

• The researchers examined the mode of action of LPA1 on lamina bending by comparing the phenotypes among knockout, overexpression and repressor-fusion transgenic plants.
• A strong synergic effect is detected between lpa1 and d2 (the defective mutant for catalysis of C-23-hydroxylated BRs) during IAA-mediated lamina inclination.

Expression

• The Ubiquitin promoter was used to overexpress LPA1 cDNA. To construct a vector expressing repressor-fused LPA1, the C-terminus of LPA1 was fused with the 24 amino acid fragment containing a conserved ERF-associated amphiphilic repression (EAR) motif of a gene (LOC_Os02g01090) orthologous to Arabidopsis SUPERMAN (At3g23130).
• RNA sequencing analysis and qRT-PCR indicate that LPA1 influences the expression of three OsPIN genes (OsPIN1a, OsPIN1c and OsPIN3a), which suggests that auxin flux might be an important factor in LPA1-mediated lamina inclination in rice.

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Labs working on this gene

• State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
• Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
• State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
• State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
• State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.