Os03g0300000

The rice Os03g0300000 was reported as OsXXT1 in 2014 by researchers from China.

Annotated Information

Figure 1. Phenotype of root hairs from wild type (WT, cv Kasalath), srh2, and srh2 complemented by Ubiquitin-1 promoter ::OsXXT1.t..

Gene Symbol

• Os03g0300000 <=> OsXXT1,XXT1,SRH2,OsSRH2

Function

• OsXXT1 in maintaining cell wall structure and tensile strength in rice, a typical grass species that contains relatively low XyG content in cell walls.

Phenotypic analysis

• Seeds from the M 2 generation of an EMS-mutagenized population of Indica cultivar Kasalath, were germinated and grown in nutrient solution to screen for mutants with abnormal root hair phenotype. Seven days after germination, a mutant with significantly reduced length in root hairs was identified (Fig. 1A, B). No obvious difference was observed in leaf or root growth between wild type and mutant (Fig. S1).
• The mutant was designated as srh2. Scanning electron microscopy of the wild-type and srh2 root surface showed that the length of the root hairs of srh2 were shorter than those of wild type.
• However, no significant difference was found in root hair density or distribution pattern between srh2 and wild type (Fig. 1D, E).

Expression

• OsXXT1 and OsGT2 have similar expression patterns and are expressed across a variety of tissues. In contrast, other members of this family (OsGT3 to OSGT5) display a more restricted expression pattern, suggesting that OsXXT1 and OsGT2 are the predominantly expressed genes.

Evolution

• OsXXT1 displays more than 70% amino acid sequence identity with the previously characterized Arabidopsis thaliana XYG XYLOSYL TRANSFERASE 1 (AtXXT1) and XYG XYLOSYL TRANSFERASE 2 (AtXXT2), which catalyse the transfer of xylose onto β-1,4-glucan chains.

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Labs working on this gene

• State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 388 Yuhangtang Road, Hangzhou, 310058, P. R. China
• University of Western Australia-Zhejiang University Joint Research Laboratory in Genomics and Nutriomics, College of Life Sciences, Zhejiang University, 388 Yuhangtang Road, Hangzhou, 310058, P. R. China
• ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley, 6009 Western Australia, Australia
• State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
• Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe

University, Bundara, Victoria 3086, Australia