Os03g0308200

The rice Os03g0308200 was reported as nrl2 in 2016 by researchers from China.

Annotated Information

Gene Symbol

• Os04g0459000 <=> NRL2,SRL2

Function

• NRL2 regulates leaf and flower development in rice.

Phenotypic analysis

• To isolate the novel gene that regulates rice leaf development, the researchers screened two indica line mutant populations that were generated by ethyl methanesulphonate (EMS) mutagenesis and identified two allelic mutants with extremely narrow and semi-rolled leaves, named nrl2-1 and nrl2-2.
• Phenotypic observation of leaf growth showed that the nrl2-1 mutant had a significantly reduced leaf blade width, attaining approximately 55.6% of the leaf width of the wild type (WT) at 70 days after sowing.
• They also found that the average number of both large veins and small veins between two adjacent large veins in the mature leaves of nrl2-1 was significantly reduced compared with WT.

Expression

• We analyzed the expression profile of NRL2 in rice using quantitative RT-PCR (qRT-PCR), which showed that the NRL2 gene displayed constitutive expression in all rice tissues and organs examined, with maximum expression in young leaves.
• We then analyzed the expression pattern of the NRL2 gene in different leaves from seedlings with three unfolded leaves. This showed that the NRL2 gene was expressed in all of the leaves examined, with maximum expression in the shoot apex region (SAR) and a gradual reduction from the SAR to the first leaf (L1).
• To determine the spatial and temporal expression patterns of NRL2 during leaf development more precisely, we performed mRNA in situ hybridization, which showed that NRL2 was particularly highly expressed in the leaf primordia, mesophyll cells, and sclerenchymatous cells.

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Labs working on this gene

• State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
• Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
• State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
• State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
• State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.