Os03g0686900

The rice Os03g0686900 was reported as FLO6 in 2014 by researchers from China.

Annotated Information

Figure 1. Phenotype of the flo6 mutant..

Gene Symbol

• Os03g0686900 <=> FLO6,OsFLO6

Function

• FLO6 encodes a ubiquitously expressed CBM48-containing protein.
• FLO6 can interact with isoamylase1 (ISA1) both in vitro and in vivo, whereas ISA1 does not bind to starch directly.
• FLO6 may act as a starch-binding protein involved in starch synthesis and compound granule formation through a direct interaction with ISA1 in developing rice seeds.

Phenotypic analysis

• The flo6 mutant, recovered from tissue culture of the japonica rice cv. Nipponbare, had no visible differences with wild-type plants during the seedling, tillering and heading stages, and mature plants were slightly shorter than wild-type plants; however, the flo6 mutant displayed a remarkably slower grain-filling rate (Figure 1a).
• This difference became apparent from approxi- mately 15 days after flowering (DAF), and was maintained until seed maturation (Figure 1a). Consistent with the lower grain-filling rate, starch synthesis in the flo6 endo- sperm was also decreased (Figure 1b).
• At the mature stage, flo6 seeds had smaller and floury endosperms (Figure 1c,e), compared with that of the wild type (Figure 1d,f).
• The interior of the endosperm in the mature grains was floury white (Figure 1f), and the SGs of the mutant grains were loosely packed (Figure 1g–j).
• Thus, the mutation in FLO6 affects starch accumulation during endo- sperm development.

Subcellular localization

• The FLO6 protein was localized to the plastid by the N–terminal transit peptide.

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Labs working on this gene

• State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China,
• College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
• National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China