Os03g0752200

The rice Os03g0752200 was reported as OsSPO11-1 in 2010 by researchers from the China.

Annotated Information

Gene Symbol

• Os03g0752200 <=>OsSPO11-1

Function

• Spo11 is a homolog of a subunit of archaebacterial topoisomerase, which catalyzes DNA double-strand breaks and initiates homologous chromosome recombination.
• OsSPO11-1 is essential for both homologous chromosomes pairing and crossover formation during meiosis in rice.

Mutation

• The researchers conducted RT-PCR to confirm that the mutant phenotype of the transgenic plants was caused by the defect of OsSPO11-1 gene. Total RNA was extracted from the prepollinated flowers of both wild type and the completely sterile transgenic plants. RT-PCR analysis showed that OsSPO11-1 transcripts in the transgenic plants were downregulated, suggesting that the sterility was caused by the knockdown of OsSPO11-1 gene in these transgenic plants.

Labs working on this gene

• Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University.
• State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,