Os04g0537900
The rice gene Os04g0537900 was reported as OsVPE1 in 2009 by researchers from China[1].
Contents
Annotated Information
Gene Symbol
- Os04g0537900 <=> glup3(esp7), esp7, glup3, OsVPE1, VPE1, OsaLeg3
Function
- OsVPE1 is a homolog of the Arabidopsis bVPE gene. OsVPE1 encodes a 497-aminoacid polypeptide.
- OsVPE1 is a cysteine protease that plays a crucial role in the maturation of rice glutelins.
Subcellular localization
Figure 8. Vacuolar targeting of the mutant C269G OsVPE1 protein. [1].
- The researchers generated two fusion proteins in which green fluorescent protein (GFP) was terminally fused with either wild-type OsVPE1 (pNVG) or mutant OsVPE1 (pWVG). These proteins were expressed in onion epidermal cells. As expected, the control GFP signals localize to both the cytoplasm and nuclei of the cells (Figure 8a). Fusion proteins expressed from both the pNVG and pWVG constructs were detected in vacuoles (Figure 8b,c).
Evolution
Figure 4. A phylogenic tree of plant vacuolar processing enzyme (VPE) proteins. The rooted tree was generated based on a multiple sequence alignment using the program DNAMAN. The scale represents the percentage substitution per site. GenBank accession numbers for each protein and their similarity to OsVPE1 are indicated. [1].
- Multiple sequence alignment shows that this VPE is more closely related to several previously identified seed-type VPEs (such as Arabidopsis bVPE, castor bean (Ricinus communis L.) VPE, rice NP1 and rice REP2) than to vegetative-type VPEs (such as AtaVPE, cVPE, dVPE and Proteinase B) (Figure 4).
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Labs working on this gene
- National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
- National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
