Os04g0607600

The rice Os04g0607600 was reported as HKT1;4 in 2016 by researchers from Japan, Italy and USA.

Annotated Information

Gene Symbol

• Os04g0459000 <=> OsHKT7,HKT7

Function

• OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress.

Expression

• Heterologous expression analysis was performed using a salt hypersensitive mutant of S. cerevisiae (strain G19).
• OsHKT1;4 expression increases Na+ hypersensitivity of yeast cells promoting Na+ over-accumulation. The OsHKT1;4 cDNA from rice cultivar Nipponbare was constitutively expressed in strain G19 of S. cerevisiae under the control of the GAP promoter.
• G19 cells harboring the empty vector or expressing OsHKT1;4 were grown on the arginine phosphate (AP) medium containing 1 mM KCl with or without the addition of 50 mM NaCl.

Phenotypic analysis

• phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress.

Subcellular localization

• To determine the localization of the OsHKT1;4 protein in plant cells, we fused EGFP at the N-terminus end of OsHKT1;4 (EGFP-OsHKT1;4 was shown to be functional in X. laevis oocytes) and placed under the control of the CaMV35S promoter.The result strongly indicated that EGFP-OsHKT1;4 localizes to the PM of rice protoplasts. However, by repeating the transformation experiments several times, we often observed that EGFP-OsHKT1;4 was also present inside the cells and clustered in punctate-like structures.

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Labs working on this gene

• State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
• Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
• State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
• State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
• State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.