Os04g0659100

The rice Os04g0659100 was reported as OsGS2 in 2000 by researchers from Japan.

Annotated Information

Gene Symbol

• Os04g0659100 <=> OsGS2, GLN2, RGS31

Function

• The rice OsGS2 can encode chloroplastic glutamine synthetase proteins.
• The rice OsGS2 is a key enzyme of assimilation process which catalyze glutamate and ammonia into glutamine at the existence of ATP.
• It is the first reaction of nitrogen assimilation. GS2 could catalyze assimilation of amino produced by chloroplasts photorespiration.
• GS2 might be involved in assimilation of ammonium derived from many sources such as nitrate in the soil, atmospheric N 2 , and endogenous amino acids as well as from photorespiration

Phenotypic analysis

• Each transgenic rice plant line showed a different accumulation level of GS2.
• A transgenic plant line, G39-2, which accumulated about 1.5-fold more GS2 than the control plant, had an increased photorespiration capacity.
• In another line, G241-12, GS2 was almost lost and photorespiration activity could not be detected.
• Fluorescence quenching analysis revealed that photorespiration could prevent the over-reduction of electron transport systems.
• When exposed to 150 mM NaCl for 2 weeks, the control rice plants completely lost photosystem II activity, but G39-2 plants retained more than 90% activity after the 2-week treatment, whereas G241-12 plants lost these activities within one week. In the presence of isonicotinic acid hydrazide, an inhibitor of photorespiration, G39-2 showed the same salt tolerance as the control plants.
• The intracellular contents of NH 4 + and Na + in the stressed plants correlated well with the levels of GS2.
• Thus, the enhancement of photorespiration conferred resistance to salt in rice plants.

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Labs working on this gene

• Research Institute, Meijo University, Nagoya, Aichi, Japan.
• Department of Chemistry, Faculty of Science and Technology, Meijo University, Tenpaku-ku, Nagoya, Aichi, 468-8502 Japan
• Plantech Research Institute, Aoba-ku, Yokohama, Kanagawa, 227-0033 Japan
• Graduate School of Bioagricultral Science, Nagoya University, Chikusa-ku, Nagoya, Aichi, 464-8601 Japan