Os04g0670900

The rice gene Os04g0670900 was reported as Sh4 in 2006 by researchers from USA.

Annotated Information

Gene Symbol

• Os04g0670900 <=> sh4, SH4, SHAT2, SHA1, qSH4, OsSh4, Sh4, Osh4

Function

• Examination of the sh4 protein, using programs Prosite and PredictNLS, identified a Myb3 DNA binding domain and a nuclear localization signal, suggesting that sh4 is a transcription factor.

• sh4 plays an important role in the establishment of the abscission layer from the early stage of flower development.

Expression

• Reverse transcription–polymerase chain reaction (RT-PCR) detected the expression of sh4 at the flower and pedicel junction, where mature grains separate from the mother plant. Gene expression was not detected in the remaining parts of flowers or pedicels or in the leaves.

Sublocalization

• The researchers fused the gene for a green fluorescent protein (GFP) with sh4 to make sh4-GFP, which was driven by a Ubi promoter in the plasmid construct. The construct was introduced into a japonica cultivar, Taipei 309, a rice strain tested as suitable for gene transformation. The nuclear localization of GFP-tagged sh4 was determined.

Evolution

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Labs working on this gene

• Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA.