Os05g0156900
The rice Os05g0156900 was reported as chalk5 in 2014 [1] by researchers from China.
Contents
Annotated Information
Figure 4. Subcellular localization and analysis of the Chalk5 protein.[1].
Gene Symbol
- Os05g0156900 <=>chalk5,OsChalk5
Function
- Chalk5 encodes a vacuolar H + -translocating pyrophosphatase (V-PPase) with inorganic pyrophosphate (PP i ) hydrolysis and H+ -translocation activity.
- Two consensus nucleotide polymorphisms in the Chalk5 promoter in rice varieties might partly account for the differences in Chalk5 mRNA levels that contribute to natural variation in grain chalkiness.
Expression
- Elevated expression of Chalk5 increases the chalkiness of the endosperm, putatively by disturbing the pH homeostasis of the endomembrane trafficking system in developing seeds, which affects the biogenesis of protein bodies and is coupled with a great increase in small vesicle-like structures, thus forming air spaces among endosperm storage substances and resulting in chalky grain.
Phenotypic analysis
- In subcellular localization analysis, both proteins encoded by the two alleles from the parents colocalized with a vacuole-specific marker (VIT1 protein), indicating vacuolar localization of the Chalk5 protein.
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Labs working on this gene
- National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
- Shanghai Agrobiological Gene Center, Shanghai, China.
References
- ↑ 1.0 1.1 Li Y, Fan C, Xing Y, Yun P, Luo L, Yan B, Peng B, Xie W, Wang G, Li X, Xiao J, Xu C, He Y. Chalk5 encodes a vacuolar H(+)-translocating pyrophosphatase influencing grain chalkiness in rice. Nat Genet. 2014 Apr;46(4):398-404. doi: 10.1038/ng.2923. Epub 2014 Mar 16. Erratum in: Nat Genet. 2014 Jun;46(6):657. PubMed PMID: 24633159.
