Os05g0341600
the rice gene Os05g0341600 was recongized as RAB21 in 1988, which is induced when plants are subject to water-stress.
Contents
Annotated Information
Gene Symbol
- Os05g0341600 <=> OsRab5D1,Rab5D1,RAB21
Function
- the rice gene Os05g0341600 was recongized as RAB21 in 1988, which is induced to balance water-stress of plants. encoding a basic, glycine-rich protein (mol.wt 16 529) which has a duplicated domain structure.
- Immunoblots probed with antibodies raised against Bgalactosidase/RAB 21 fusion protein detect RAB 21 protein only in cytosolic cell fractions. [1][1][2][3]
Expression
- RAB 21 mRNA and protein accumulate in rice embryos, leaves, roots and callus-derived suspension cells upon treatment with NaCl (200 mM) and/or the plant hormone abscisic acid (10 /tMABA).
- The effects of NaCI and ABA are not cumulative, suggesting that these two inducers share a common response pathway. Induction of RAB 21 mRNA accumulation by ABA is rapid (< 15 min in suspension cells) and does not require protein synthesis, indicating that preformed nuclear and/or cytosolic factors mediate the response to this hormone.
- the reseachers have characterized the RAB 21 gene by deternmining the complete nucleotide sequence of a nearly full-length cDNA and corresponding genomiccopy, and by mapping the start site of its major transcript. The proximal promoter region contains various GC-rich repeats.
Knowledge Extension
- The hormone abscisic acid (ABA) mediates a number of important physiological processes in plants (King, 1976; Jones et al., 1987).
- Developmental studies have shown that ABA induces the accumulation of specific mRNAs and proteins late during embryogenesis in seeds of diverse species (Galau et al., 1986; Finklestein et al., 1985; Litts et al.,1987).
- At this time, the level of endogenous ABA increases,the seeds desiccate and the embryos of some species become dormant (King, 1976; Suzuki et al., 1981).
- In cereal seeds,some of the 'late' developmental mRNAs are long-lived in mature, dry grains, but are rapidly degraded during seed germination.
- However, exogenously applied ABA causes the precocious accumulation of these mRNAs in immature embryos and their reappearance in germinating seeds (Finklestein et al., 1985; Mundy et al., 1986).
Labs working on this gene
- Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
References
- ↑ 1.0 1.1 Mundy, J. and N.-H. Chua (1988). "Abscisic acid and water-stress induce the expression of a novel rice gene." The EMBO Journal 7(8): 2279.
- ↑ Bray EA, Beachy RN. Regulation by ABA of beta-Conglycinin Expression in Cultured Developing Soybean Cotyledons. Plant Physiol. 1985 Nov;79(3):746–750.
- ↑ Finkelstein RR, Tenbarge KM, Shumway JE, Crouch ML. Role of ABA in Maturation of Rapeseed Embryos. Plant Physiol. 1985 Jul;78(3):630–636.
