Os05g0365300
The rice Os05g0365300 was reported as DEPG1 in 2012 [1] by researchers from China.
Contents
Annotated Information
Figure 3. Phylogenetic analysis of DEPG1 with the 18 NBS-LRR R genes based on their amino acid sequences.[1].
Figure 5. Histological analysis of DEPG1 expression in transgenic plants.[1].
Gene Symbol
- Os05g0365300 <=> DEPG1,OsRP1L1,OsBBR1,rNBS33
Function
- DEPG1 is a nucleotide-binding site (NBS)-leucine rich repeat (LRR) gene, and the deduced protein sequence of DEPG1 has approximately 64% identity with that of the disease resistance gene Pi37.
Expression
- Semi-quantitative PCR assays showed that DEPG1 is widely expressed in rice, and is preferentially expressed in internodes, leaf blades, leaf sheaths and flag leaves.
- Observation of cross sections of leaves from the transgenic plants with a DEPG1-promoter::glucuronidase (GUS) fusion gene revealed that DEPG1 is also highly expressed in mesophyll tissues where Xoc mainly colonizes.
- Xoc negatively regulates expression of DEPG1 at the early stage of the pathogen infection, and so do the three defense-signal compounds including salicylic acid (SA), methyl jasmonate (MeJA) and 1-aminocyclopropane-1-carboxylic-acid (ACC).
Phenotypic Analysis
- Transgenic rice plants overexpressing DEPG1 exhibit enhanced susceptibility to Xoc compared to the wild-type controls.
- Enhanced susceptibility to Xoc may be mediated by inhibition of the expression of some SA biosynthesis-related genes and pathogenesis-related genes that may contribute to the disease resistance.
- DEPG1 plays roles in the interactions between rice and BLS pathogen Xoc.
Subcellular Location
- DEPG1 protein is located to the cytoplasm, which was confirmed by transient expression of DEPG1-GFP (green fluorescent protein) fusion construct in onion epidermal cells.
Evolution
- Phylogenetic analysis of DEPG1 and the 18 characterized NBS-LRR genes revealed that DEPG1 is more closely related to Pi37.
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Labs working on this gene
- Xiamen Key Laboratory for Plant Genetics, School of Life Sciences, Xiamen University, Xiamen, 361005, People's Republic of China.
References
- ↑ 1.0 1.1 1.2 Guo L, Li M, Wang W, Wang L, Hao G, Guo C, Chen L. Over-expression in the nucleotide-binding site-leucine rich repeat gene DEPG1 increases susceptibility to bacterial leaf streak disease in transgenic rice plants. Mol Biol Rep. 2012 Apr;39(4):3491-504. doi: 10.1007/s11033-011-1122-6. Epub 2011 Jun 30. PubMed PMID: 21717056.
