Os05g0446000
The rice gene Os05g0446000 was reported as DNG701 in 2011[1] by researchers from USA and China.
Contents
Annotated Information
Gene Symbol
- Os05g0446000 <=> DNG701,OsDNG701
Function
Fig. 1. Enzymatic activity of DNG701 on 5-meC-containing DNA substrates in vitro. [1].
- DNG701 encodes a large (1,812 amino acid residues) DNA glycosylase domain protein.
- Rice DNG701 is a 5-meC DNA glycosylase/lyase responsible for the demethylation of Tos17 and this DNA demethylase plays a critical role in promoting Tos17 transposition in rice calli.
Mutation
- Knockout or knockdown of DNG701 in rice plants led to DNA hypermethylation and reduced expression of the retrotransposon Tos17. Tos17 showed less transposition in calli derived from dng701 knockout mutant seeds compared with that in wild-type calli.
Overexpression
- Overexpression of DNG701 in both rice calli and transgenic plants substantially reduced DNA methylation levels of Tos17 and enhanced its expression. The overexpression also led to more frequent transposition of Tos17 in calli.
Expression
Evolution
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Labs working on this gene
- Department of Plant Pathology, Ohio State University, Columbus, OH 43210;
- Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907;
- Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711; *Department of Biochemistry and Molecular Biology, Mississippi State University, MS 39762;
- Center for Plant Stress Genomics Research, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia;
- State Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
References
- ↑ 1.0 1.1 La H, Ding B, Mishra GP, Zhou B, Yang H, Bellizzi Mdel R, Chen S, Meyers BC, Peng Z, Zhu JK, Wang GL. A 5-methylcytosine DNA glycosylase/lyase demethylates the retrotransposon Tos17 and promotes its transposition in rice. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15498-503. doi: 10.1073/pnas.1112704108. PubMed PMID: 21896764; PubMed Central PMCID: PMC3174586.
