Os06g0142350

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The rice Os06g0142350 was reported as OsENOD93-1 in 2009 [1] by researchers from Canada.

Annotated Information

Function

  • Development of genetic varieties with improved nitrogen-use efficiency (NUE) is essential for sustainable agriculture.
  • The rice OsENOD93-1 is an early nodulin gene with positive effects on nitrogen-use efficiency.
  • The OsENOD93-1 gene responded significantly to both N induction and N reduction.

Phenotypic analysis

  • Transgenic rice plants over-expressing the OsENOD93-1 gene had increased shoot dry biomass and seed yield.
  • Transgenic plants accumulated higher concentrations of total amino acids and total N in roots.
  • A higher concentration of amino acids in xylem sap was detected in transgenic plants, especially under N stress.

Expression

  • The OsENOD93-1 gene was expressed at high levels in roots of wild-type (WT) plants
  • In situ hybridization revealed that OsENOD93-1 is expressed in vascular bundles, as well as in epidermis and endodermis.

Subcellular localization

  • The OsENOD93-1 protein product was localized in mitochondria.

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Labs working on this gene

  • Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada N1G 2W1,
  • Syngenta Biotechnology, Inc., Research Triangle Park, NC 27709, USA,
  • Department of Genetics, Fudan University, Shanghai 200433
  • Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada N1G 2W1

References

  1. Bi YM, Kant S, Clarke J, Gidda S, Ming F, Xu J, Rochon A, Shelp BJ, Hao L, Zhao R, Mullen RT, Zhu T, Rothstein SJ. Increased nitrogen-use efficiency in transgenic rice plants over-expressing a nitrogen-responsive early nodulin gene identified from rice expression profiling. Plant Cell Environ. 2009 Dec;32(12):1749-60. doi: 10.1111/j.1365-3040.2009.02032.x. Erratum in: Plant Cell Environ. 2010 Feb;33(2):304. Clark, Joseph [corrected to Clarke, Joseph]. PubMed PMID: 19682292.

Structured Information