Os06g0213100
The rice gene Os06g0213100 was reported as S5 in 2008[1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os06g0213100 <=> S5,OsS5
Function
- S5 encodes an aspartic protease conditioning embryo-sac fertility.
- The S5 Gene Encodes an AP.
- The indica (S5-i) and japonica (S5-j) alleles differ by two nucleotides.
Fig. 1. Fertility of ORF5-NJ11 transgenic-positive and -negative plants with Balilla as the recipient. [1].
Expression
- Expression of S5 is extremely low throughout the life cycle, only at the background level in almost all of the stages.
Sublocalization
- Posttranslational transport and activation of APs involves complex processing, which makes it difficult to determine the subcellular localization of S5 by using a transgenic approach by fusing the protein with a reporter. We used immunogold labeling and transmission electron microscopy (TEM) techniques to assay the subcellular localization of S5, using sections of nucellar tissues where the gene showed relatively high expression. It was revealed that the gold particles were distributed in large quantity in the cell walls, whereas in very small quantity in other subcellular compartments. Thus, the final destination of this protein is the cell wall, consistent with the nature of a secreted protein as predicted by the signal peptide.
Evolution
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Labs working on this gene
- National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China;
- Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China;
- Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
- National Center of Crop Design, China Bioway Biotech Group, Beijing 100085, China;
- College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China;
- National Maize Improvement Center, China Agriculture University, Beijing 100094, China
References
