Os06g0348800
The rice Os06g0348800 was reported as OsGLK1 in 2009 [1] by researchers from Japan.
Contents
Annotated Information
Gene Symbol
- Os06g0348800 <=> OsGLK1,GLK1
Function
- OsGLK1 regulates chloroplast development under the control of light and phytohormones, and that it is a key regulator of chloroplast development.
Phenotypic analysis
- The rice expression-microarray and northern blot analyses, showd that a large number of nucleus-encoded genes involved in chloroplast functions were highly expressed and transcripts of plastid-encoded genes, psaA , psbA and rbcL , increased in the OsGLK1-FOX calli.
- Transmission electron microscopy showed the existence of differentiated chloroplasts with grana stacks in OsGLK1 -FOX calli cells.
- However, in darkness, OsGLK1-FOX calli did not show a green color or develop grana stacks.
- Developed chloroplasts was found in vascular bundle and bundle sheath cells of coleoptiles and leaves from OsGLK1-FOX seedlings.
- The OsGLK1-FOX calli exhibited high photosynthetic activity and were able to grow on sucrose-depleted media, indicating that developed chloroplasts in OsGLK1-FOX rice calli are functional and active.
Expression
- the endogenous OsGLK1 mRNA level was observed to be increased synchronously with the greening of wild-type calli after transfer to plantlet regeneration medium.
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Labs working on this gene
- Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602 Japan
- Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602 Japan
- Department of Applied Biological Chemistry, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan
References
- ↑ Nakamura H, Muramatsu M, Hakata M, Ueno O, Nagamura Y, Hirochika H, Takano M, Ichikawa H. Ectopic overexpression of the transcription factor OsGLK1 induces chloroplast development in non-green rice cells. Plant Cell Physiol. 2009 Nov;50(11):1933-49. doi: 10.1093/pcp/pcp138. PubMed PMID: 19808806; PubMed Central PMCID: PMC2775961.
