Os06g0625400

The rice gene Os06g0625400 was reported as spp in 2010 by researchers from China.

Annotated Information

Gene Symbol

• Os06g0625400 <=> spp, Osspp

Function

• SPP encodes a metalloendopeptidase that cleaves a broad range of precursor substrates.
• SPP is essential for chloroplast biogenesis at the early growth stage and for rice root development

Mutation

• spp mutant shows leaf chlorosis at the early seedling stage but inhibition of root growth during the whole growth period.
• The expression of some genes associated with chloroplast development was decreased in young seedlings of the spp mutant, but not in 14-day-old seedlings. Western blot analysis revealed that the Rubisco small subunit is not precisely processed in the spp mutant in 7-day-old seedlings, but the processing activity in the spp mutant is restored in 14-day-old seedlings.
• Moreover, the expression levels of Cab1R and Cab2R for the light-harvesting chlorophyll a / b -binding protein (LHCP) were highly up-regulated in the transgenic plants with overexpression of SPP.

Expression

• Semi-quantitative RT–PCR analysis was performed to investigate the expression pattern of SPP in different tissues. The results showed that SPP was expressed in all the tested tissues, with a similar level in the spp mutant and the WT, and with higher expression levels in chloroplast-containing green tissues. SPP mRNAs also accumulated in roots. No signifi cant change in transcript levels was observed in the WT and the spp mutant shoots of 7- and 14-day-old plants. Furthermore, no differences in SPP mRNAs were observed when the WT and spp mutant plants were grown under light and dark conditions.

Sublocalization

• Subcellular localization analysis indicates that SPP is targeted to the chloroplast.

Evolution

You can also add sub-section(s) at will.

Labs working on this gene

• State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, PR China