Os07g0103500
The rice gene Os07g0103500 was reported as GA2ox5 in 2008[1] by researchers from China and USA.
Contents
Annotated Information
Gene Symbol
- Os07g0103500 <=> GA2ox5, OsGA2ox5
Function
- GA2ox5 is a member of the rice (Oryza sativa) GA2ox family which regulat plant growth by controling GA levels during flowering, tillering, and seed germination.
Mutation
- The semidwarf mutant M27337, designated as GA2ox5D335- 341ACT, carries a T-DNA insertion in the coding region, at a position 23 bp upstream of the translation stop codon of GA2ox5 (Figure 5B). Truncation of GA2ox5 by T-DNA resulted in a loss of seven amino acids at the C terminus of the putative GA2ox5 polypeptide. Accumulation of the truncated GA2ox5 mRNA was significantly enhanced by T-DNA activation tagging in both homozygous and heterozygous mutants, but the semidwarf phenotype was observed only in the homozygous mutant. The GA2ox5D335-341ACT homozygous mutant had an average plant height of 90% and produced seeds with an average fertility of 88% of the wild type.
Figure 5. Severely Dwarfed and Semidwarfed Rice Mutants Obtained by T-DNA Activation Tagging.(B) The semidwarf mutant GA2ox5D335-341ACT (M27337). [1].
Expression
Figure 3. Differential Expression of Two Groups of GA2oxs Regulates Flower and Tiller Development. [1].
- To understand the role that individual GA2oxs may play in rice growth, their temporal expression profiles during the rice life cycle were examined. As leaves have been shown to be a major site of GA biosynthesis (Choi et al., 1995), mRNAs were purified from leaves at different growth stages ranging from 5 to 100 d after imbibition (DAI) and analyzed by RT-PCR. Genes GA2ox1 to GA2ox9 were differentially expressed in leaves, and their expression was also temporally regulated (Figure 3).
Evolution
- Amino acid sequence comparison generated a phylogenetic tree among the rice GA2ox family and 19 GA2oxs from eight dicot plant species, which revealed that rice GA2ox5, GA2ox6, and GA2ox9 are more closely related to the Arabidopsis GA2ox7 and GA2ox8 and spinach GA2ox3 (Figure 2).
Figure 2. Phylogenetic Tree Based on the Comparison of Plant GA2oxs. [1].
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Labs working on this gene
- Institute of Molecular Biology, National Chung-Hsing University, Taichung 402, Taiwan, Republic of China
- Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan, Republic of China
- Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan, Republic of China
- Department of Energy Plant Research Laboratory and Department of Plant Biology, Michigan State University,
East Lansing, Michigan 48824-1312
References
