Os07g0124700

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The rice Os07g0124700 was reported as CRL5 in 2011 [1] by researchers from Japan.

Annotated Information

Gene Symbol

  • Os07g0124700 <=> 'OsPLT8, CRL5, AP2/EREBP141, AP2/EREBP#141

Function

  • Cytokinin is known to have negative effects on de novo auxin-induced root formation. However, the regulatorymechanisms of root initiation by both cytokinin and auxin are poorly understood.
  • CRL5 encodes a member of the APETALA2 (AP2)/EHYLENE RESPONSIVE FACTOR (ERF) transcription factor family.[1]
  • CRL5 functions downstream of the AUXIN (AUX)/INDOLE-3-ACETIC ACID (IAA) and AUXIN RESPONSE FACTOR (ARF)-mediated auxin signaling pathways involved in crown root initiation. [1]
  • Further analysis revealed that CRL5 upregulates a type-A response regulator(RR) of cytokinin signaling, OsRR1. [1]
  • It indicated that auxin-induced CRL5 functions as a positive regulator of crown root initiation through repression of cytokinin signaling.[1]
  • Schematic diagram of the de novo root initiation. CRL5 may function in de-differentiated cells (highlighted) to promote root initiation, not in the de-differentiation process. The factor inducing the de-differentiation is unknown(finger.g)[2]

Evolution

  • Through a BLAST search using the amino acid sequence of CRL5, there are two homologous genes in rice, CRL5-like1 and CRL5-like2, which shared 57 and 49% amino acid identity,respectively, with CRL5.[1]
  • Comparison of the amino acid sequences of two AP2 domains and the conserved intervening linker region in CRL5, CRL5-like1 and CRL5-like2 from rice, and ANT, PLT1 and PLT2 from Arabidopsis.[1]
  • CRL5 shares significant sequence similarity with AINTEGUMENTA (ANT) containing 2 AP2 domains in Arabidopsis.[3][2]
  • Seven AINTEGUMENTA-like (AIL)/PLETHORA (PLT) proteins were reported to share high sequence similarity with ANT.[4][2][5]

Expression

  • The expression of CRL5, which encodes a member of the large AP2/ERF transcription factor family protein, was observed in the stem region where crown root initiation occurs. Exogenous auxin treatment induced CRL5 expression without de novo protein biosynthesis, which also required the degradation of AUX/IAA proteins.[1]

Subcellular localization

  • Localized GFP signal of ProCRL5:GFP in a crown root tip (b), crown root with emerging lateral roots (c), lateral root primordium (d) and stem base of the plant.[1]
  • In situ hybridization with cross sections through the nodes in a 7-day-old wild-type plant. Localization of CRL5 in the regions where crown root initiation occurs (the identical region shown in fb and g), as determined using the antisense (i) and sense probes (j). No signal was detected with the sense probe of CRL5. The red arrowhead indicates the peripheral cylinder of vascular bundles. Scale bars = 200 lm.(Figure 3i,j).[1]

Phenotypic analysis

  • Although wild-type plants (cv. Kinmaze) formed many crown roots by the seedling stage, the number of crown roots produced by crl5 was significantly reduced at the identical stage of development (Figure a,f).
  • Comparison of lateral root number between wild-type and crl5. ns, not significant(Figure g).
  • Comparison of a seminal root length between wild-type and crl5. *, Statistically significant difference from wild-type (P < 0.05)(Figure h).
  • It has been reported that a reduction of crown root number commonly occurs in auxin-related mutants in rice[6][7][8][9].
  • An abnormality in gravitropic response is also a characteristic auxin-related phenotype in both rice and Arabidopsis[10][11].

Labs working on this gene

  • Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi 464-8601, Japan,
  • Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi 464-8601, Japan, and
  • Institute for Advanced Research, Nagoya University, Nagoya, Aichi 464-8601, Japan

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Kitomi Y, Ito H, Hobo T, et al. The auxin responsive AP2/ERF transcription factor CROWN ROOTLESS5 is involved in crown root initiation in rice through the induction of OsRR1, a type‐A response regulator of cytokinin signaling[J]. The Plant Journal, 2011, 67(3): 472-484.
  2. ↑ 2.0 2.1 2.2 Kitomi Y, Kitano H, Inukai Y. Molecular mechanism of crown root initiation and the different mechanisms between crown root and radicle in rice[J]. Plant signaling & behavior, 2011, 6(9): 1276.
  3. ↑ Elliott RC, Betzner AS, Huttner E, Oakes MP, Tucker WQ, Gerentes D, et al. AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth.Plant Cell 1996; 8:155-68; PMID:8742707; DOI:10.1105/tpc.8.2.155.
  4. ↑ Nole-Wilson S, Tranby TL, Krizek BA.AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or divisioncompetent states. Plant Mol Biol 2005; 57:613-28; PMID:15988559; DOI:10.1007/s11103-005-0955-6.
  5. ↑ Inukai Y, Sakamoto T, Ueguchi-Tanaka M, et al. Crown rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin signaling[J]. The Plant Cell Online, 2005, 17(5): 1387-1396.
  6. ↑ Inukai, Y., Sakamoto, T., Ueguchi-Tanaka, M., Shibata, Y., Gomi, K., Umemura, I., Hasegawa, Y., Ashikari, M., Kitano, H. and Matsuoka, M. (2005) Crown Rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin Signaling. Plant Cell, 17, 1387–1396.
  7. ↑ Liu, H., Wang, S., Yu, X., Yu, J., He, X., Zhang, S., Shou, H. and Wu, P. (2005) ARL1, a LOB domain protein required for adventitious root ormation in rice. Plant J. 43, 47–56.
  8. ↑ Kitomi, Y., Ogawa, A., Kitano, H. and Inukai, Y. (2008) CRL4 regulates crown root formation through auxin transport in rice. Plant Root, 2, 19–28.
  9. ↑ Liu, S., Wang, J., Wang, L., Wang, X., Xue, Y., Wu, P. and Shou, H. (2009) Adventitious root formation in rice requires OsGNOM1 and is mediated by the OsPINs family. Cell Res. 19, 1110–1119.
  10. ↑ Leyser, H.M., Pickett, F.B., Dharmasiri, S. and Estelle, M. (1996) Mutations in the AXR3 gene of Arabidopsis result in altered auxin response including ectopic expression from the SAUR-AC1 promoter. Plant J. 10, 403–413.
  11. ↑ Tian, Q. and Reed, J.W. (1999) Control of auxin-regulated root development by the Arabidopsis thaliana SHY2/IAA3 gene. Development, 126, 711–721.

Structured Information

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